PurposeRetroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71445||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesH. sapiens (human)
Entrez GeneSTAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLEGFP-Y705F-STAT3 was a gift from George Stark (Addgene plasmid # 71445 ; http://n2t.net/addgene:71445 ; RRID:Addgene_71445)
For your References section:Critical role for lysine 685 in gene expression mediated by transcription factor unphosphorylated STAT3. Dasgupta M, Unal H, Willard B, Yang J, Karnik SS, Stark GR. J Biol Chem. 2014 Oct 31;289(44):30763-71. doi: 10.1074/jbc.M114.603894. Epub 2014 Sep 12. 10.1074/jbc.M114.603894 PubMed 25217633