174,565 results
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Plasmid#221565PurposeExpress OpenCRISPR-1 nuclease in human cells using a CMV promoterDepositorInsertOpenCRISPR-1
TagsNLSExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
gag-PE
Plasmid#211371PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-PE
ExpressionMammalianAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
ExpressionBacterialAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-HKRA-sgRNA_v7
Plasmid#208357PurposeExpression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-HKRA
ExpressionMammalianMutationI123H/D195K/D208R/V232APromoterCMVAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD-p19
Plasmid#196326Purpose35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressorDepositorInserttomato bushy stunt virus p19
ExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAF0687 Pa01_attP-mCherry donor
Plasmid#193466PurposePromoterless mcherry donor for Pa01DepositorInsertPa01_attP
ExpressionMammalianAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG2a-Tn5
Plasmid#184288PurposeAnti-mouse IgG2a Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG2a-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon DREADD Gi-mCherry
Plasmid#177672PurposeExpresses DREADD hM4D (Gi) in Cre and Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianPromoternEFAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
TagsGST and his6ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP StrepII
Plasmid#160686PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTags3C eGFP StrepIIExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-CAG-SARS-CoV2-Spike D614G-HA
Plasmid#158761Purpose3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M PaoDepositorInsertSARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
UseLentiviralTagsHAExpressionMammalianMutationSpike D614GPromoterCAGAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianPromoterHuman Synapsin-1Available SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0
Plasmid#153285PurposeExpress the adenosine sensor GRAB_Ado1.0 in neuronsDepositorHas ServiceAAV1InsertAdenosine sensor GRAB_Ado1.0
UseAAVPromoterhSynAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-8his
Plasmid#149268PurposeMammalian expression plasmid for his-tagged sACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA4mt
Plasmid#140463PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertR-CEPIA4mt
TagsMycExpressionMammalianAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SV40LT
Plasmid#136616PurposeDox-inducible lentiviral vector expressing SV40 large T antigen for immortalizing cellsDepositorInsertSV40 large T antigen
UseLentiviralExpressionBacterialAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only