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Axel Brunger Lab Plasmids


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Title Authors Publication
Central pore residues mediate the p97/VCP activity required for ERAD. DeLaBarre B, Christianson JC, Kopito RR, Brunger AT Mol Cell. 2006 May 19. 22(4):451-62.
Structural basis of FFAT motif-mediated ER targeting. Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT Structure. 2005 Jul . 13(7):1035-45.
Structures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Arac D, Boucard AA, Ozkan E, Strop P, Newell E, Sudhof TC, Brunger AT Neuron. 2007 Dec 20. 56(6):992-1003.
A potent peptidomimetic inhibitor of botulinum neurotoxin serotype A has a very different conformation than SNAP-25 substrate. Zuniga JE, Schmidt JJ, Fenn T, Burnett JC, Arac D, Gussio R, Stafford RG, Badie SS, Bavari S, Brunger AT Structure. 2008 Oct 8. 16(10):1588-97.
A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. Arac D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Sudhof TC, Brunger AT EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26.
Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis. Zhou Q, Lai Y, Bacaj T, Zhao M, Lyubimov AY, Uervirojnangkoorn M, Zeldin OB, Brewster AS, Sauter NK, Cohen AE, Soltis SM, Alonso-Mori R, Chollet M, Lemke HT, Pfuetzner RA, Choi UB, Weis WI, Diao J, Sudhof TC, Brunger AT Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 2015 Aug 17.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12281pBD-0005VCP K315L (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12339pBD-0071VCP K251A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12344pBD-0010VCP E305Q (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12345pBD-0016VCP R313K (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp 3110001E05, CDC48, p97, p97/VCP Add to Cart
12346pBD-0013VCP R313A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12347pBD-0006VCP T316V (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12348pBD-0009VCP T317Q (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12349pBD-0070VCP H317N (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12350pBD-0069VCP H317A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12351pBD-0008VCP E319L (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12352pBD-0007VCP E319Q (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12353pBD-0018VCP R322A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12354pBD-0020VCP R322K (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12355pBD-0022VCP R323A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12356pBD-0024VCP R323K (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12357pBD-0032VCP R362A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12358pBD-0035VCP W551A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12359pBD-0039VCP F552A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12360pBD-0037VCP W551A F552A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12361pBD-0072VCP E578Q (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12362pBD-0050VCP R586A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12363pBD-0003VCP R586K (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12364pBD-0026VCP D592N (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12365pBD-0028VCP D598N (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12366pBD-0045VCP R599A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12367pBD-0011VCP R599K (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12368pBD-0030VCP R638A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12369pBD-0082VCP K524A (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp Add to Cart
12373pBD-0001VCP (Mus musculus)Bacterial ExpressionCentral pore residues mediate the p97/VCP activity required for ERAD. Mol Cell. 2006 May 19. 22(4):451-62. pET-28a Vcp 3110001E05, CDC48, p97, p97/VCP Add to Cart
13395pGEX-T-rnVAPA-1-219VAP-A (Rattus norvegicus)Bacterial ExpressionStructural basis of FFAT motif-mediated ER targeting. Structure. 2005 Jul . 13(7):1035-45. pGEX-4T Vapa Add to Cart
13396pGEX-T-rnVAPA-1-125VAP-A (Rattus norvegicus)Bacterial ExpressionStructural basis of FFAT motif-mediated ER targeting. Structure. 2005 Jul . 13(7):1035-45. pGEX-4T Vapa Add to Cart
18874pEGFP-N1-VAPA(1-242)VAP-A (Rattus norvegicus)Mammalian ExpressionStructural basis of FFAT motif-mediated ER targeting. Structure. 2005 Jul . 13(7):1035-45. pEGFP-N1 Vapa Add to Cart
18875pEGFP-N1-VAPA(1-242) K87D/M89DVAP-A (Rattus norvegicus)Mammalian ExpressionStructural basis of FFAT motif-mediated ER targeting. Structure. 2005 Jul . 13(7):1035-45. pEGFP-N1 Vapa Add to Cart
20172rNL1(46-638)Neuroligin 1 (Rattus norvegicus)Insect ExpressionStructures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Neuron. 2007 Dec 20. 56(6):992-1003. pACGP67a Nlgn1 Add to Cart
20173rNrx1beta(47-299)Neurexin 1 beta (Rattus norvegicus)Insect ExpressionStructures of neuroligin-1 and the neuroligin-1/neurexin-1 beta complex reveal specific protein-protein and protein-Ca2+ interactions. Neuron. 2007 Dec 20. 56(6):992-1003. pACGP67a Nrxn1 Nrxn1b Add to Cart
31602BoNT/A-LCBoNT/A LCBacterial ExpressionA potent peptidomimetic inhibitor of botulinum neurotoxin serotype A has a very different conformation than SNAP-25 substrate. Structure. 2008 Oct 8. 16(10):1588-97. pBN3 Add to Cart
37847pAcGP67A-rCL1-HormR-GAINCL1 (Rattus norvegicus)Baculovirus Transfer VectorsA novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. pAcGP67A Adgrl1 CL1BA, Lphn1 Add to Cart
37848pAcGP67A-hBAI3-HormR-GAINBAI3 (Homo sapiens)Baculovirus Transfer VectorsA novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. pAcGP67A BAI3 KIAA0550, MGC133100 Add to Cart
70055pDC184His tagged Synaptobrevin 28-89 (Rattus norvegicus), Syntaxin 191-256 (Rattus norvegicus)Bacterial ExpressionArchitecture of the synaptotagmin-SNARE machinery for neuronal exocytosis. Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 2015 Aug 17. Co-expresses Syt1-SNARE37aa-linker complex with p045 pACYCDuet-1 Add to Cart
70057p045C2AB-linker-SNAP-25_C (Rattus norvegicus), SNAP-25_N (Rattus norvegicus)Bacterial ExpressionArchitecture of the synaptotagmin-SNARE machinery for neuronal exocytosis. Nature. 2015 Sep 3;525(7567):62-7. doi: 10.1038/nature14975. Epub 2015 Aug 17. Co-expresses Syt1-SNARE37aa-linker complex with pDC184 pETDuet-1 Add to Cart