167,150 results

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pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Promoter

anhydrotetracycline Ptet promoter

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163043

Purpose

QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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CIp10 - γmGFP (γmGFP based)

Plasmid

#163119

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

gamma mGFP

Use

Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.eGFP.WPRE.bGH

Plasmid

#105539

Purpose

AAV expression of EGFP from hSyn promoter

Depositor

James M. Wilson

Has Service

AAV1

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKT-PH[R25C]-GFP

Plasmid

#18837

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

R25C

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA-GFP

Plasmid

#215489

Purpose

Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Parkin

Plasmid

#187897

Purpose

Mammalian cell expression of untagged Parkin

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

PRKN (PRKN Human)

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-EGFP

Plasmid

#177136

Purpose

Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

EGFP

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMS_366

Plasmid

#182131

Purpose

expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

Depositor

George Church

Article

Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

Inserts

retron RNA
ec.86 RT
CspRecT

Mutation

incorporated rifampicin-resistance-conferring don…

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP1-Venus

Plasmid

#234750

Purpose

Mammalian expression of lysosome marker LAMP1-Venus

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

LAMP1-Venus

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-ConVERGD-eGFP-W3SL

Plasmid

#218748

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

CAG

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Tags

His-tagged

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP46

Plasmid

#22584

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP46 (USP46 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pBUE411-GGB

Plasmid

#198233

Purpose

Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411

Depositor

Andrea Gallavotti

Article

Chen et al bioRxiv 2022.09.02.506370

Inserts

GRF4-GIF1 chimera cassette
pPLTP::BBM cassette

Use

CRISPR; Plant binary vector

Expression

Plant

Promoter

Ubiquitin and maize PLTP

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pMMTV neu NT

Plasmid

#1823

Depositor

Philip Leder

Article

Muller et al Cell 1988 Jul 1;54(1):105-15.

Insert

neu NT (Erbb2 Rat)

Expression

Mammalian

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only