169,216 results

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTK92_Lifeact-GFP

Plasmid

#46356

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Lifeact

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX

Plasmid

#111516

Purpose

Expresses E. coli ClpX with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1- dCas9-2xNLS-EGFP

Plasmid

#74710

Purpose

Expresses dCas9-2xNLS-EGFP in mammalian cells

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

dCas9-2xNLS-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRham-CALM1

Plasmid

#182500

Purpose

Bacterial expression of the Homo sapiens calmodulin, CALM1

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

CALM1 (CALM1 Human)

Expression

Bacterial

Promoter

rhamnose

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-hYAP1

Plasmid

#17843

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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pCN-mBFP

Plasmid

#63847

Purpose

Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Insert

mBFP

Expression

Bacterial

Mutation

Promoter and RBS was swapped. His-tag was removed…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHR EGFR-CD3ε-FusionRed

Plasmid

#188626

Purpose

Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Human, Synthetic)

Use

Lentiviral

Tags

CD3ε-FusionRed

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry-PH

Plasmid

#36075

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

PH domain of PLCdelta1 (PLCD1 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLGB31

Plasmid

#126621

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and rhamnose-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

Plasmid

#223249

Purpose

Mammalian expression of the optimized R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor_opt

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-HA-ATXN3

Plasmid

#22538

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

ATXN3 (ATXN3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_pGK_mCherry

Plasmid

#79124

Purpose

Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NLRC4

Plasmid

#60199

Purpose

Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NLRC4 (Nlrc4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

Plasmid

#182390

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
OmpA-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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p246 eSpCas9_2gRNAs_mH11

Plasmid

#164854

Purpose

gRNA vector for targeting mouse H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

gRNAs for targeting mouse H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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