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PurposeIntegration of luciferase operon in Tn7 insertion site of Gram negative bacteria
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Depositing Lab
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Depositing OrganizationINRA Nouzilly
Located in France -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 69150-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 69150-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGRG25
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Backbone manufacturerNancy Craig - Johns Hopkins University (Addgene Plasmid #16665)
- Backbone size w/o insert (bp) 12500
- Total vector size (bp) 19671
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameluxCDABE
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SpeciesPhotorhabdus luminescens
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Insert Size (bp)6200
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GenBank IDAF403784
- Promoter frr
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer AATTGCCCGTCGTATTAAAG
- 3′ sequencing primer GTAGCGTCGTAAGCTAATAC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 69150-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 69150-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEN276 was a gift from Pierre Germon (Addgene plasmid # 69150 ; http://n2t.net/addgene:69150 ; RRID:Addgene_69150) -
For your References section:
Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon. Howe K, Karsi A, Germon P, Wills RW, Lawrence ML, Bailey RH. BMC Microbiol. 2010 Jul 23;10:197. doi: 10.1186/1471-2180-10-197. 10.1186/1471-2180-10-197 PubMed 20653968
Map uploaded by the depositor.
