pEF6-CSPG4-myc-his
(Plasmid #69037)

• Purpose: Expression of CSPG4 in mammalian cells
• Depositing Lab: Wensheng Wei
• Publication: Yuan et al Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014 Dec 30.

Backbone

• Vector backbone: pEF6-Myc-His-B
  (Search Vector Database)
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 5802
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CSPG4
• Alt name: MCSP; NG2; HMW-MAA
• Species: H. sapiens (human)
• Insert Size (bp): 6966
• Mutation: none
• GenBank ID: 1464
• Entrez Gene: CSPG4 (a.k.a. CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2)
• Promoter: EF-1 alpha
• Tags / Fusion Proteins:
  • Myc (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Spe I (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

BstX I, EcoRI, and EcoRV sites were destroyed during cloning