165,075 results

1. 4XCLEAR-luciferase reporter

   Plasmid

   #66800

   Purpose

   Reporter for activity of the CLEAR network

   Depositor

   Albert La Spada

   Article

   Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

   Insert

   4XCLEAR
   

   Use

   Luciferase

   Promoter

   Four CLEAR elements (GTCACGTGAC) in tandem derive…

   Available Since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

   Plasmid

   #74928

   Purpose

   Translation reporter

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   24xGCN4_v4-kif18b-24xPP7
   

   Expression

   Mammalian

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTet-IRES-EGFP

   Plasmid

   #64238

   Purpose

   Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

   Depositor

   Maria Lung

   Article

   Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   pTet

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 VDAC1-TC

   Plasmid

   #211733

   Purpose

   Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.

   Depositor

   Roland Malli

   Article

   Pilic et al bioRxiv 2023.09.08.556841

   Insert

   Voltage-dependent anion channel 1 (VDAC1 )
   

   Tags

   Tetracysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-SFXN1

   Plasmid

   #132311

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SFXN1 (SFXN1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP-N1-IL6

   Plasmid

   #111933

   Purpose

   Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

   Insert

   Interleukin 6 (IL6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   CMV

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-SARS-CoV-2-S-RBD-8his

   Plasmid

   #145145

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
   

   Tags

   8his tag

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGCP123-EbpA_g1

   Plasmid

   #153517

   Purpose

   sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

   Depositor

   Kimberly Kline

   Article

   Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

   Insert

   pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGreenFire1_E2F1RE

    Plasmid

    #112248

    Purpose

    expression of GFP and Luciferase under the control of E2F1 responsive element

    Depositor

    Simone Di Giovanni

    Insert

    E2F1RE (responsive element)
    

    Use

    Lentiviral

    Tags

    copGFP and luciferase

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57KAN-40XALFA

    Plasmid

    #233118

    Purpose

    40X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Mutation

    None

    Available Since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57KAN-20XALFA

    Plasmid

    #233112

    Purpose

    20X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Mutation

    None

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Expression

    Mammalian

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-HA-Nedd4/2795

    Plasmid

    #11426

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Nedd4-1 (Nedd4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D661N compared to GenBank reference sequence U966…

    Promoter

    CMV

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-tdTomato-CNA35

    Plasmid

    #61606

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    tdTomato and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid

    #178091

    Purpose

    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHyg-AID*-6HA

    Plasmid

    #99520

    Purpose

    C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    6xHA

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 8xNFAT-ZsG-hCD4

    Plasmid

    #162746

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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