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pUK21
(Plasmid #49788)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49788 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC21 (Addgene plasmid #49787)
  • Backbone manufacturer
    Messing Lab
  • Backbone size (bp) 3090
  • Modifications to backbone
    The bla gene of pUC21 was replaced with the Kanamycin resistance gene from Tn903 by cutting pUC21 with BspHI, filling in the ends, and inserting the Tn903 fragment. The HindIII and SmaI sites have been removed from the Kanamycin Resistance gene so that these sites within the MCS are now unique and can be used for cloning.
  • Vector type
    cloning vector
  • Promoter lacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUK21 was a gift from Joachim Messing (Addgene plasmid # 49788 ; http://n2t.net/addgene:49788 ; RRID:Addgene_49788)
  • For your References section:

    New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Vieira J, Messing J. Gene. 1991 Apr;100:189-94. 10.1016/0378-1119(91)90365-I PubMed 1905257