|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49788||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC21 (Addgene plasmid #49787)
Backbone manufacturerMessing Lab
- Backbone size (bp) 3090
Modifications to backboneThe bla gene of pUC21 was replaced with the Kanamycin resistance gene from Tn903 by cutting pUC21 with BspHI, filling in the ends, and inserting the Tn903 fragment. The HindIII and SmaI sites have been removed from the Kanamycin Resistance gene so that these sites within the MCS are now unique and can be used for cloning.
Vector typecloning vector
- Promoter lacZ
Growth in Bacteria
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUK21 was a gift from Joachim Messing (Addgene plasmid # 49788 ; http://n2t.net/addgene:49788 ; RRID:Addgene_49788)
For your References section:New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Vieira J, Messing J. Gene. 1991 Apr;100:189-94. 10.1016/0378-1119(91)90365-I PubMed 1905257