|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49789||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC21 (Addgene plasmid #49787)
Backbone manufacturerMessing Lab
- Backbone size (bp) 2135
Modifications to backboneThe lac region and MCS are identical to pUC21, The KmR gene is from Tn905 and the ori is P15A from pACYC 177.
Vector typecloning vector
- Promoter lacZ
Growth in Bacteria
Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOK12 was a gift from Joachim Messing (Addgene plasmid # 49789 ; http://n2t.net/addgene:49789 ; RRID:Addgene_49789)
For your References section:New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Vieira J, Messing J. Gene. 1991 Apr;100:189-94. 10.1016/0378-1119(91)90365-I PubMed 1905257