166,289 results

1. pLB

   Plasmid

   #11619

   Purpose

   pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

   Depositor

   Stephan Kissler

   Article

   Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-dTAG-Zeo

   Plasmid

   #207738

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-Ef1a-DIO EYFP

   Plasmid

   #27056

   Depositor

   Karl Deisseroth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. JD633

   Plasmid

   #160393

   Purpose

   CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Inserts

   ZmUbi::wheat GRF4-GIF1 chimera
   ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   Binary vector

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3165

   Plasmid

   #144641

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

   Plasmid

   #203908

   Purpose

   Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   Gal4UAS_dGFP_PGK_iRFP
   

   Expression

   Mammalian

   Promoter

   5xUAS and PGK

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-DEST47 PINK1 C-GFP

   Plasmid

   #13316

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

   Plasmid

   #162278

   Purpose

   Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

   Depositor

   Al George

   Article

   DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

   Insert

   SCN1A Variant 1, stabilized (SCN1A Human)
   

   Tags

   IRES-mScarlet

   Expression

   Mammalian

   Mutation

   IVS intron inserted after bp2946. Silent codon c…

   Promoter

   CMV

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-RNF2

    Plasmid

    #126544

    Purpose

    Expresses GFP-tagged RNF2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RING1B (RNF2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lentiviral mBmal1-Luc

    Plasmid

    #182762

    Purpose

    Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

    Depositor

    Qing-Jun Meng

    Article

    Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

    Insert

    mouse arntl promoter (Bmal1 Mouse)
    

    Use

    Lentiviral

    Promoter

    649 bp

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hc-MYC

    Plasmid

    #17220

    Purpose

    Retroviral expression of human cMYC

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.10-Claudin-1

    Plasmid

    #46387

    Purpose

    Claudin-1 promoter reporter assay

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    CLDN1 Promoter (CLDN1 Human)
    

    Use

    Luciferase

    Promoter

    CLDN1 promoter

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

14. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GAL4UAS

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. RXFP3-Tango

    Plasmid

    #66494

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP3 (RXFP3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLG010 (DRT type 2)

    Plasmid

    #157888

    Purpose

    Expresses the DRT type 2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 2
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPGKPhiC31obpA

    Plasmid

    #13795

    Purpose

    Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

    Insert

    PhiC31o
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXJ-Myc-IRF2

    Plasmid

    #232642

    Purpose

    transient expresses IRF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF2 (IRF2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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