164,577 results

1. pcDNA3 flag p53

   Plasmid

   #10838

   Depositor

   Thomas Roberts

   Article

   Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS1375

   Plasmid

   #29176

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple88 (GFAP Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. mTFP1-N1

   Plasmid

   #54521

   Purpose

   Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AP4mS_PPVs_P3_cLuc

   Plasmid

   #119300

   Purpose

   Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_FASN

   Plasmid

   #107138

   Purpose

   His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Insert

   Fatty acid synthase (FASN Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-FOXA2

   Plasmid

   #153110

   Purpose

   Expression of Flag tagged human FOXA2 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXA2 (FOXA2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT13araE

   Plasmid

   #18987

   Depositor

   Jay Keasling

   Article

   Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

   Insert

   araE (araE E.coli)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pENTR TERRA

   Plasmid

   #111383

   Purpose

   TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   TERRA
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4-UPRE-luc2P-Hygro

    Plasmid

    #101788

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xUPRE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP1-N1-Dsup-deltaC

    Plasmid

    #90023

    Purpose

    To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal region

    Promoter

    CMV

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPHR-mCherry-NLS

    Plasmid

    #103819

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-mCherry-NLS (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMY-MCS-P2A-PuroR-HA

    Plasmid

    #163352

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGMC00018 (aka pRC0082)

    Plasmid

    #195320

    Purpose

    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19 PKC alpha

    Plasmid

    #8429

    Depositor

    Frederic Mushinski

    Insert

    Mouse Protein Kinase C alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

16. A*68:01:01

    Plasmid

    #203292

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*68:01:01 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIBN-EYFP-hPMLIII

    Plasmid

    #103808

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Use

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Promoter

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge