162,160 results

1. pST39-pNL29

   Plasmid

   #91696

   Purpose

   Expresses Mega GVs in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

   Insert

   Mega GV gene cluster (from pNL29) in pST39 backbone
   

   Use

   Tags

   No tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.0-TERT G228A

   Plasmid

   #84926

   Purpose

   luciferase reporter for mutant TERT promoter

   Depositor

   Joseph Costello

   Article

   Bell et al Science. 2015 May 29;348(6238):1036-9. d

   Insert

   TERT promoter (TERT Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   G228A

   Promoter

   TERT

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIPZ shcGAS-Mmu

   Plasmid

   #127698

   Purpose

   Doxycyclin inducible shRNA knockdown of mouse cGAS gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shcGAS Mmu (Cgas Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA1015

   Plasmid

   #189589

   Purpose

   T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   T7gp1.7 Locus 250bp Homology Arms
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Encodes for a full deletion of T7gp1.7

   Promoter

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

   Plasmid

   #122147

   Purpose

   Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mApple-TOMM20-N-10

   Plasmid

   #54955

   Purpose

   Localization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

   Plasmid

   #203228

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Insert

   Membrane-localized optopatch for neuronal dendrites
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330S-2

   Plasmid

   #58778

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGE32

    Plasmid

    #63159

    Purpose

    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Rice snoRNA U3 promoter for gRNA/PTG expression a…

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL0M-S-mTurquoise2-EC18152

    Plasmid

    #137074

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDM-Tat1b

    Plasmid

    #164442

    Purpose

    Encodes HIV-1 Tat accessory protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    HIV-1 Tat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Mm.cargo(Cre)

    Plasmid

    #174862

    Purpose

    Expresses mouse Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by MmPeg10 UTRs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hsyn-NE2m

    Plasmid

    #208686

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

    Depositor

    Yulong Li

    Insert

    GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

    Plasmid

    #173886

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG:: mCherry-2a-hM4Dnrxn

    Plasmid

    #52523

    Purpose

    Co-expresses hM4Dnrxn and a red fluorescent label

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    mCherry-2a-hM4Dnrxn
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAg

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHIV_pTp66p51

    Plasmid

    #78233

    Purpose

    Expresses HIV reverse transcriptase

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Inserts

    p66
    p51
    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Const. Promoter (From DNA2.0 Vec) and pTac Minima…

    Available since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKIR1.1

    Plasmid

    #85758

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.3_SOX2

    Plasmid

    #26817

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-SOX2-3'UTR (SOX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge