166,289 results

1. pCMV-BE2

   Plasmid

   #73020

   Purpose

   Expresses BE2 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM047 [T7-assisted CROPseq sgRNA expression vector]

   Plasmid

   #224899

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

   Depositor

   Thomas Norman

   Article

   Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC28-BIRC3

   Plasmid

   #196366

   Purpose

   protein expression of the His-tagged full length protein

   Depositor

   Stefan Knapp

   Insert

   BIRC3/cIAP2
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.hSynap.iGABASnFR

   Plasmid

   #112159

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Has Service

   AAV1

   Insert

   iGABASnFR
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   July 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PBCAG-eGFP

   Plasmid

   #40973

   Depositor

   Joseph Loturco

   Article

   Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

   Inserts

   PiggyBac transposon 3' terminal repeats
   PiggyBac 5' terminal repeats
   

   Expression

   Mammalian

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-cofilin WT

   Plasmid

   #78295

   Purpose

   Mammalian expression of mouse cofilin fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRXIP 3xFLAG-VMP1

   Plasmid

   #117416

   Purpose

   Expresses VMP1 tagged with 3xFLAG in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   VMP1 (VMP1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. S96 ELP

   Plasmid

   #68393

   Purpose

   Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S96 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)

   Plasmid

   #231346

   Purpose

   Self complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.

   Depositor

   Viviana Gradinaru

   Article

   Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

   Insert

   T7/T3-BC(p1-14)
   

   Use

   AAV

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1A-CD19.28H.28TM.BB.3z

    Plasmid

    #200679

    Purpose

    Modular CD28hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD28HD-CD28TM-41BB-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. haA1.pBABEpu

    Plasmid

    #21813

    Depositor

    David Ron

    Article

    Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

    Insert

    GADD34 C-term
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. Watermelon backbone

    Plasmid

    #155258

    Purpose

    Can be used to generate a Watermelon library

    Depositor

    Joan Brugge, Aviv Regev

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4-ERSE2-luc2P-Hygro

    Plasmid

    #101790

    Purpose

    Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

    Depositor

    Seiichi Oyadomari

    Article

    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

    Insert

    3xERSE-II
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. VSVG-mCherry-RUSH

    Plasmid

    #203647

    Purpose

    RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

    Depositor

    Juan Bonifacino

    Article

    Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

    Insert

    VSVG
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pc DNA FRT TO- APPSwed/Ind

    Plasmid

    #114194

    Purpose

    Creation of a stable cell line expressing APP mutation (AD in vitro study)

    Depositor

    Aleksandra Radenovic

    Insert

    Amyloid-Precursor-Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    Popular mouse model, overexpress 2 mutations: Swe…

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid

    #50476

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(-) HuTF-Fc-His tag

    Plasmid

    #183253

    Purpose

    Soluble expression of the extracellular domains of human tissue factor (TF)-Fc

    Depositor

    Alexander McLellan

    Insert

    Tissue factor (F3 Human)
    

    Tags

    8xHis

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-hSOX2

    Plasmid

    #17218

    Purpose

    Retroviral expression of human SOX2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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