165,075 results

1. pEGFP-IRAlus-Nicn1

   Plasmid

   #92345

   Purpose

   Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX304-IRES-Td

   Plasmid

   #176296

   Purpose

   pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-TLR7-YFP

   Plasmid

   #13022

   Depositor

   Doug Golenbock

   Insert

   TLR7 (TLR7 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-cfGFP

   Plasmid

   #200773

   Purpose

   Expresses cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   cysteine-free GFP
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKL2299

   Plasmid

   #186332

   Purpose

   This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

   Depositor

   Kan Wang

   Article

   Kang et al Front Plant Sci. 2022 May 4;13:860971. d

   Insert

   virulence genes from Agrobacterium tumefaciens pTiBo542
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-EF1aH N90-beta-catenin

   Plasmid

   #86499

   Purpose

   Mammalian expression of Myc tagged N90 beta-catenin

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-90; constitutively acti…

   Promoter

   EF1a

   Available Since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasAb-apr

   Plasmid

   #121998

   Purpose

   Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GAL-ccdB

   Plasmid

   #14147

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Gateway destination

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1-GfaABC1D-lck-jGCaMP8s

   Plasmid

   #176761

   Purpose

   AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Has Service

   AAV1 and AAV9

   Insert

   lck-jGCaMP8s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GfaABC1D

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV anti-FITC-LaG16-Y186A-T2A-BFP

    Plasmid

    #194932

    Purpose

    LV construct encoding Y186A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_Lifeact_HaloTag9

    Plasmid

    #175545

    Purpose

    Expression of HaloTag9 at the actin filaments of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SrtAdelta59

    Plasmid

    #51138

    Purpose

    expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

    Depositor

    Hidde Ploegh

    Article

    Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

    Insert

    S.aureus SrtA delta 59
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pENTR221-BNT162b2ΔUTR

    Plasmid

    #171214

    Purpose

    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

    Depositor

    Liang Wei Wang

    Article

    Low et al medRxiv 2021.04.27.21256151

    Insert

    BNT162b2ΔUTR
    

    Use

    Gateway cloning

    Mutation

    Kozak sequence changed from CCCGCCACC to GGCTGCAC…

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDragon-p21

    Plasmid

    #155016

    Purpose

    For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

    Depositor

    Alberto Rosello-Diez

    Article

    Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1-sh-mSOX9-5

    Plasmid

    #40646

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSOX9-5 (Sox9 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Linear-GFP

    Plasmid

    #226258

    Purpose

    Production of linear RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MS2-epegRNA-Dnmt1

    Plasmid

    #211372

    Purpose

    Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MS2-epegRNA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCAG EGFP

    Plasmid

    #14857

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2002 Feb 19. 9

    Insert

    EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 11, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pBAD-D2

    Plasmid

    #37470

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D2 second generation cameleon (calcium sensor)
    

    Expression

    Bacterial

    Promoter

    Ara

    Available Since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pmal_c5x_RNAse_Inhib

    Plasmid

    #153314

    Purpose

    Bacterial expression of an RNase inhibitor

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    RNAse Inhibitor
    

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Available Since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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