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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mGreenLantern

Plasmid

#164468

Purpose

Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

AAV

Tags

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

EF1a

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s ONE-GO

Plasmid

#189731

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-s ONE-GO
GNAS (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pFloat.cspA_v1

Plasmid

#133071

Purpose

Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

Depositor

David Sabatini, Kacper Rogala

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Mutation

Promoter

cspA

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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ALDH18A1

Plasmid

#155402

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ALDH18A1 (ALDH18A1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSCIP-hCD69-TdTomato

Plasmid

#154095

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Inserts

[5HA]-P2A-tdTomato-[3HA]
hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pACS141

Plasmid

#204597

Purpose

Histone H3.3-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human histone H3.3 (H3-3A Human)

Use

Tags

human histone H3.3 - V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_9 [pUB110 ORI]

Plasmid

#198924

Purpose

Level 0 part

Depositor

Nili Ostrov, Charlie Gilbert, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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mVenus N1

Plasmid

#27793

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Has Service

Cloning Grade DNA

Insert

mVenus N1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSK-mPar1

Plasmid

#53227

Purpose

bacterial expression of mouse Par1

Depositor

Shaun Coughlin

Insert

Par1 (F2r Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only
lenti.Cas9.BFP.Blast

Plasmid

#196714

Purpose

WT SpCas9 with BSD-TagBFP for selection and sorting

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSNAPf-H2B Control Plasmid

Plasmid

#101124

Purpose

Expression of SNAP-tagged Histone H2B in mammalian cells

Depositor

Ana Egana, New England Biolabs

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Use

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Use

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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GEPIR Sox4.2137

Plasmid

#101119

Purpose

shRNA Vector

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

Sox4.2137

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM3

Plasmid

#58397

Purpose

Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (IFITM3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

Plasmid

#198753

Purpose

Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

See depositor comments below

Promoter

EF1a

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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GEPIR Sox4.2091

Plasmid

#101118

Purpose

shRNA vec

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

Sox4.2137

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only