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Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation.
Fuentes DR, Swigut T, Wysocka J
Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989. PubMed Article

Plasmids from Article

ID Plasmid Purpose
191269PiggyBac dCas9-VPRpiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP
191314PiggyBac dCas9-KRABpiggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP
191316LTR5HS S. pyogenes scaffold CARGO arrayContains an array of guide RNAs targeting LTR5Hs elements
191317LTR5HS S. aureus scaffold CARGO arrayContains an array of guide RNAs targeting LTR5Hs elements
191319nontarget S. pyogenes scaffold CARGO arrayContains an array of nontargeting guide RNAs

Antibodies from Article