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PiggyBac dCas9-VPR
(Plasmid #191269)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191269 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 191269-D50 50 μg of DNA in Tris buffer $495
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DNA 191269-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    piggyBac
  • Total vector size (bp) 15639
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VPR-dCas9-HA-tagBFP
  • Species
    Synthetic; Streptococcus pyogenes
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • tagBFP (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    rtTA3-IRES-PURO
  • Species
    Synthetic
  • Insert Size (bp)
    1939

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was cloned by Dr. Daniel Fuentes.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac dCas9-VPR was a gift from Joanna Wysocka (Addgene plasmid # 191269 ; http://n2t.net/addgene:191269 ; RRID:Addgene_191269)

  • For your References section:

    Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation. Fuentes DR, Swigut T, Wysocka J. Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989. 10.7554/eLife.35989 PubMed 30070637
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