Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing.
Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, Banfield J, Doudna JA
Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 10.1038/s41564-022-01258-x. Epub 2022 Oct 31. PubMed Article
Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, Banfield J, Doudna JA
Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 10.1038/s41564-022-01258-x. Epub 2022 Oct 31. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186235 | pBA559 | pTet-LbuCas13a crRNA entry vector |
| 186236 | pBA560 | pTet-eLbuCas13a crRNA entry vector |
| 186237 | pBA562 | pTet-RfxCas13d crRNA entry vector |
| 186238 | pBA707 | KanR-BBR1 entry vector for HR donor |
| 186239 | pBA615 | eLbuCas13a Negative Control (RFP targeting) |
| 186240 | pBA620 | LbuCas13a Negative Control (RFP targeting) |
| 186241 | pBA630 | RfxCas13d Negative Control (RFP targeting) |
| 186242 | pBA787 | soc-C HR Donor Vector (T4 phage editing) |
| 186243 | pBA788 | soc-S HR Donor Vector (T4 phage editing) |
| 186244 | pBA789 | soc-F HR Donor Vector (T4 phage editing) |
| 186245 | pBA790 | dnap-C HR Donor Vector (T4 phage editing) |
| 186246 | pBA791 | dnap-S HR Donor Vector (T4 phage editing) |
| 186247 | pBA792 | dnap-F HR Donor Vector (T4 phage editing) |
| 189580 | pBA1032 | T4soc deletion HR vector 250bp HR Arms (T4 phage editing) |
| 189582 | pBA1030 | EdH4gp0004 deletion HR vector 250bp HR Arms (EdH4 phage editing) |
| 189584 | pBA1031 | EdH4gp0214 deletion HR vector 250bp HR Arms (EdH4 phage editing) |
| 189587 | pBA1018 | T4wtGT7 deletion HR vector 250bp HR Arms (T4 phage editing) |
| 189589 | pBA1015 | T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing) |