Rc/CMV cyclin D3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||10912||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5542
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecyclin D3
SpeciesH. sapiens (human)
Insert Size (bp)2000
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (destroyed during cloning)
- 3′ cloning site BstXI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
D3 cloned in as klenow'ed EcoRI fragment at BstXI site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Rc/CMV cyclin D3 was a gift from Bob Weinberg (Addgene plasmid # 10912 ; http://n2t.net/addgene:10912 ; RRID:Addgene_10912)
For your References section:Regulation of retinoblastoma protein functions by ectopic expression of human cyclins. Hinds PW, Mittnacht S, Dulic V, Arnold A, Reed SI, Weinberg RA. Cell. 1992 Sep 18. 70(6):993-1006. 10.1016/0092-8674(92)90249-C PubMed 1388095