161,547 results

1. pdRBFC-B2-YN

   Plasmid

   #74721

   Purpose

   Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

   Depositor

   Aiming Wang

   Article

   Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

   Insert

   Influenza A virus NS1 protein, partial
   

   Use

   Double-stranded rna labeling

   Tags

   HA and YFP N-terminal half domain

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-hSyn-RFP

   Plasmid

   #22909

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Has Service

   Cloning Grade DNA

   Insert

   human synapsin I promoter driving DsRedExpress (SYN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-HA-SAMTOR

   Plasmid

   #100513

   Purpose

   Expresses HA tagged SAMTOR in mammalian cells

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   SAMTOR (BMT2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-SRT-tdTomato

   Plasmid

   #154885

   Purpose

   piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   piggyBac SRT
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP VAMP7 (1-220)

   Plasmid

   #42316

   Depositor

   Thierry Galli

   Article

   Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

   Insert

   VAMP7 (1-220) (VAMP7 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRDA_052

   Plasmid

   #136474

   Purpose

   Lentiviral expression of AsCas12a gRNA

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   Puromycin resistance
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ChlorON-1

   Plasmid

   #193477

   Purpose

   Turn-on fluorescent sensor for chloride

   Depositor

   Sheel Dodani

   Article

   Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

   Insert

   ChlorON-1
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MX1_pCSdest

   Plasmid

   #53951

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   MX1 (MX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. PIK3C2B gRNA (BRDN0001149054)

   Plasmid

   #76712

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pdRBFC-B2-YC

    Plasmid

    #74722

    Purpose

    Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    FLAG and YFP C-terminal half domain

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-f:PGC1

    Plasmid

    #1026

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGTM_YR375_SUMO_Protease_001

    Plasmid

    #190063

    Purpose

    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    ULP1_YEAST (ULP1 Budding Yeast)
    

    Use

    Tags

    8X His-MBP (Maltose Binding Protein)

    Expression

    Bacterial

    Mutation

    Construct contains residues 347 – 621

    Promoter

    T7 lac

    Available since

    Jan. 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCI-EGFP-NR2b wt

    Plasmid

    #45447

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Promoter

    Available since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEV-G3-Km

    Plasmid

    #46817

    Purpose

    TEF1 and GAL10 promoter controlled expression cassettes with G418 resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and GAL10

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PIK3C2B gRNA (BRDN0001149112)

    Plasmid

    #76713

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161927

    Purpose

    Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine repression reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.10-VEGFprom(-1000 to -1)

    Plasmid

    #66128

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    VEGF -1000 to -1

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge