165,567 results

1. pFRT-TODestFLAGHA_HuC

   Plasmid

   #65756

   Purpose

   expresses FLAGHA-tagged HuC in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL3 (ELAVL3 Human)
   

   Tags

   FLAG HA and V5

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

   Plasmid

   #112021

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta/alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Sept. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Neil Fairweather, Robert Fagan

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-TDP-43ΔNLS-mClover3

   Plasmid

   #214916

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-HA-USP33

   Plasmid

   #22601

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP33 (USP33 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO-neo (1795)

   Plasmid

   #41000

   Depositor

   Jonathon Pines

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Klc1-GFP

   Plasmid

   #186617

   Purpose

   Encodes Kinesin Light Chain 1 fused to GFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Kinesin Light Chain 1 (KLC1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC475 - pAAV c-fos iRFP

   Plasmid

   #47906

   Purpose

   An AAV vector that expresses iRFP under the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   c-fos

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #125692

   Purpose

   Global labeling of inhibitory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3254

    Plasmid

    #144730

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGH8 - pRAB-3::mCherry::unc-54utr

    Plasmid

    #19359

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Prab-3::mCherry
    

    Tags

    mCherry

    Expression

    Worm

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPRv2-sgCTRL

    Plasmid

    #209750

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    Cas9
    

    Use

    Lentiviral

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-pur H-2Kb

    Plasmid

    #111623

    Purpose

    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

    Depositor

    Jon Yewdell

    Insert

    H2Kb
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSGKP-spe

    Plasmid

    #117234

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. enIscB-T5E

    Plasmid

    #205411

    Purpose

    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    

    Expression

    Mammalian

    Mutation

    E85R+H369R+S387R+S457R

    Promoter

    CAG, hU6, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221_EGFP

    Plasmid

    #25899

    Purpose

    Gateway entry vector containing EGFP.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Tags

    EGFP and His6

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-mEB1-mCherry-6xHis

    Plasmid

    #130984

    Purpose

    Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-mCherry-6xHis (Mapre1 Mouse)
    

    Tags

    mCherry-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWZL Blast GFP

    Plasmid

    #12269

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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