161,641 results

1. pCDNA4/TO-GFP-SPOP (WT)

   Plasmid

   #197008

   Purpose

   Mammalian inducible expression vector for GFP-tagged SPOP

   Depositor

   Tanja Mittag

   Article

   Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   -

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRDX1A-c001

   Plasmid

   #175473

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX1 (PRDX1 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG87 FAST

   Plasmid

   #130719

   Purpose

   Expresses His-tagged FAST (also called YFAST) in bacteria

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PhyB-mCherry-PEX3

   Plasmid

   #51572

   Purpose

   Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   PEX3 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD649-HAsp-TNFRSF12A-COMP5AP-AviTag-9xHis

   Plasmid

   #157052

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TNFRSF12A (TNFRSF12A Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

   Plasmid

   #202606

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-3XFLAG-EGFP-OMP25

   Plasmid

   #83354

   Purpose

   For tagging mitochondria with Sigma FLAG epitopes

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XFLAG-EGFP-OMP25
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-synapsin-WHaloCaMP1a

   Plasmid

   #205308

   Purpose

   Production of AAV particles encoding WHaloCaMP1a from the synapsin promoter

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLM-mCherry-Klf4

    Plasmid

    #23243

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKH115_GALNT2-keima

    Plasmid

    #214969

    Purpose

    Golgiphagy flux reporter GALNT2-keima

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    GALNT2-keima (GALNT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSaGuide

    Plasmid

    #64710

    Purpose

    Cloning and expression of Staphylococcus aureus guide RNA

    Depositor

    Kiran Musunuru

    Insert

    Staphylococcus aureus guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

    Plasmid

    #170712

    Purpose

    dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Inserts

    mNeonGreen
    mScarlet
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TNNT2 and aMHC

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. M13KO7-ΔPS-ΔgeneIII

    Plasmid

    #134351

    Purpose

    Helper phage for M13 phage production

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Helper phage gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBA904

    Plasmid

    #122238

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Tet-O-FUW-EGR3-P2A-mCherry

    Plasmid

    #203861

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. eGFP-proα2(I)

    Plasmid

    #119826

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-5 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. dCas9-GCN5 core

    Plasmid

    #179551

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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