166,664 results

1. TMEM16F-M522K-peGFP-N1

   Plasmid

   #228579

   Purpose

   Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Expression

   Mammalian

   Mutation

   M522K plus a 3 amino acid N-terminal truncation (…

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET iGluSnFR3 v857

   Plasmid

   #175186

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v857
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB CAG-mTagBFP2-T2A-iCreERT2

   Plasmid

   #177308

   Purpose

   CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox; Piggybac

   Expression

   Mammalian

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-puro-mIR-31

   Plasmid

   #26088

   Depositor

   Bob Weinberg

   Article

   Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

   Insert

   miR-31 (MIR31 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

5. GSK3beta  (1015)

   Plasmid

   #49491

   Purpose

   expresses human Gsk3beta

   Depositor

   Scott Friedman

   Article

   Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

   Insert

   GSK3beta (GSK3B Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-PB-PGK-tetR-KRAB-2A-Puro

   Plasmid

   #129536

   Purpose

   PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

   Depositor

   Hideyuki Okano

   Article

   Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

   Insert

   PB-PGK-tetR-KRAB-2A-Puro
   

   Use

   Unspecified

   Promoter

   hPGK promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

   Plasmid

   #141194

   Purpose

   Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

   Insert

   MAP3K20 (MAP3K20 Human)
   

   Tags

   Strep, HA

   Expression

   Mammalian

   Mutation

   Changed Lysine 45 to Alanine

   Promoter

   CMV

   Available Since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-Ataxin3Q84

   Plasmid

   #22123

   Depositor

   Henry Paulson

   Article

   Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Plasmid

   #175777

   Purpose

   pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHelper_TS_V2_ampR

    Plasmid

    #214467

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Promoter

    XylS / AraC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-NR2B

    Plasmid

    #17925

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl D-aspartate receptor 2B (Grin2b Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. AcGFP-MSH6

    Plasmid

    #215123

    Purpose

    Expresses AcGFP-MSH6 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH6 (MSH6 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Rab5CA(Q79L)

    Plasmid

    #35138

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Constitutively Active- Q79L

    Promoter

    CMV

    Available Since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR2.1 Clover-LMNA Donor

    Plasmid

    #122508

    Purpose

    Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)
    

    Use

    Homology repair template plasmid (donor plasmid)

    Tags

    Clover

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_ID2_WT_V5

    Plasmid

    #82960

    Purpose

    Gateway Donor vector containing ID2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ID2 (ID2 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-1 and dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEXfrt-mCherry-KASH

    Plasmid

    #209200

    Purpose

    Flp-dependent expression of mCherry tagged KASH protein domain

    Depositor

    Larry Zweifel

    Insert

    mCherry-KASH
    

    Use

    AAV

    Tags

    KASH localization domain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA Myc DBC1

    Plasmid

    #35096

    Depositor

    Osamu Hiraike

    Article

    Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

    Insert

    deleted in breast cancer 1 (CCAR2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 Flag beta-2-adrenergic-receptor

    Plasmid

    #14697

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B2AR (ADRB2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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