179,844 results

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCW57.1-DUX4-WT

Plasmid

#99282

Purpose

All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

Depositor

Stephen Tapscott

Publication

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDIRECT_23C

Plasmid

#91140

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-N1_hTRIM32

Plasmid

#69541

Purpose

Expresses human TRIM32 fused to c-terminal EGFP

Depositor

Martin Dorf

Publication

Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tripartite motif containing 32 (TRIM32 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFC333

Plasmid

#87844

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
ble (bleomycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT7-VP7SA11

Plasmid

#89167

Purpose

Express Rotavirus SA11 VP7 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 VP7

Tags

*see below

Expression

Mammalian

Promoter

T7 promotor

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223_FBXW7_WT

Plasmid

#81795

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

V600E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT7-NSP5SA11

Plasmid

#89172

Purpose

Express Rotavirus SA11 NSP5 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 NSP5

Expression

Mammalian

Promoter

T7 promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Publication

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mini singlet oxygen generator 2

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SarA_P1-mCherry from pRN11

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPaint-TagGFP2-PuroR

Plasmid

#80970

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagGFP2

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTriEx-mCherry-Zdk1

Plasmid

#81057

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Zdk1

Tags

6x His tag, FLAG, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Publication

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Publication

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FU- dio PSD95-mCherry- W

Plasmid

#73919

Purpose

Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Publication

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

PSD-95-mCherry

Use

Lentiviral

Tags

mCherry and myc

Expression

Mammalian

Promoter

Ubi-C

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pClneoMyc-LATS1

Plasmid

#66851

Purpose

Express Myc-tagged LATS1 in mammalian cells

Depositor

Yutaka Hata

Publication

Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LATS1 (LATS1 Human)

Tags

Myc

Expression

Mammalian

Mutation

P44L mutation in LATS1 compared to GenBank refere…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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paGFP-Omp25

Plasmid

#69598

Purpose

expresses GFP-labeled mitochondrial outer membrane protein 25

Depositor

David Sabatini

Publication

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

paGFP-Omp25 (Synj2bp Rat)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

photoactivatable GFP fused to rat Omp25 C-terminus

Promoter

CMV

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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