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p146

Plasmid

#186178

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 146

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBSFlp

Plasmid

#45470

Depositor

Michele Swanson

Article

Bryan et al Appl Environ Microbiol. 2011 Apr;77(7):2

Insert

Flp recombinase

Expression

Bacterial

Promoter

T7

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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SBE-Luc

Plasmid

#16527

Depositor

Bert Vogelstein

Article

Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Insert

Smad binding element

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
GRB2-V5

Plasmid

#139631

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

GRB2 (GRB2 Human)

Tags

3xV5-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-iLOVf

Plasmid

#63723

Purpose

E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

Depositor

Brian Smith

Type

Empty backbone

Tags

TEE, iLOV, 6xHis, thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-tdTomato

Plasmid

#83029

Purpose

expresses tdTomato fluorescent protein under the CAG promoter

Depositor

Angelique Bordey

Article

Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

Insert

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-hCdt1(1-100)ΔCy

Plasmid

#193759

Purpose

Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

mCherry-hCdt1(1-100)ΔCy (CDT1 Human)

Use

Lentiviral

Mutation

human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

Promoter

EF1a

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_3xFlagEzh2 WT

Plasmid

#173717

Purpose

WT EZH2 expression plasmid with 3XFLAG tag

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 (EZH2 Human)

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-NFE2L2-Puro

Plasmid

#181919

Purpose

Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

Depositor

Scott Dixon

Article

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L2 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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CD-MPR-GFP-RUSH

Plasmid

#202797

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEA186

Plasmid

#187874

Purpose

Empty pVS1-based INTEGRATE vector with mCherry cargo

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pQUAST-frt-stop-frt-p65AD::CaM

Plasmid

#64716

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Inserts

p65AD::CaM
frt-stop-frt

Expression

Insect

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGRG36

Plasmid

#16666

Depositor

Nancy Craig

Article

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::MCS

Expression

Bacterial

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pLifeAct-mScarlet3_N1

Plasmid

#189767

Purpose

Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

LifeAct-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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8061-E33

Plasmid

#234270

Purpose

Gateway ORF Entry clone of mouse Smad3 with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

Smad3 (Smad3 Mouse)

Use

Gateway Cloning

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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attP-puro donor

Plasmid

#182139

Purpose

attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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CamKII-ADAM23-pHluorin

Plasmid

#185539

Purpose

Expresses ADAM23-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

ADAM23 (Adam23 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-LACTB-dAPEX2

Plasmid

#117179

Purpose

Plasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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