Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEGFP-N1_hTRIM32
(Plasmid #69541)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69541 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4692
  • Total vector size (bp) 6659
  • Modifications to backbone
    Inserted between XhoI and BamHI sites: GCCACC [Kozak seq] - [Trim32 CDS, 1959 bp] - CG [to preserve frame].
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tripartite motif containing 32
  • Alt name
    TRIM32
  • Alt name
    HT2A; BBS11; TATIP; LGMD2H
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1967
  • GenBank ID
    NM_012210.3 http://www.ncbi.nlm.nih.gov/gene/22954
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xhoi (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CGTGTACGGTGGGAGGTCT (pEGFP-N1 For) or CMV forward
  • 3′ sequencing primer TCCAGCTCGACCAGGATG (pEGFP-N1 Rev)
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1_hTRIM32 was a gift from Martin Dorf (Addgene plasmid # 69541 ; http://n2t.net/addgene:69541 ; RRID:Addgene_69541)
  • For your References section:

    TRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymerase. Fu B, Wang L, Ding H, Schwamborn JC, Li S, Dorf ME. PLoS Pathog. 2015 Jun 9;11(6):e1004960. doi: 10.1371/journal.ppat.1004960. eCollection 2015 Jun. PPATHOGENS-D-15-00349 [pii] PubMed 26057645