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(Plasmid #69541)


Item Catalog # Description Quantity Price (USD)
Plasmid 69541 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4692
  • Total vector size (bp) 6659
  • Modifications to backbone
    Inserted between XhoI and BamHI sites: GCCACC [Kozak seq] - [Trim32 CDS, 1959 bp] - CG [to preserve frame].
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    tripartite motif containing 32
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    TRIM32 (a.k.a. BBS11, HT2A, LGMD2H, LGMDR8, TATIP)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xhoi (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CGTGTACGGTGGGAGGTCT (pEGFP-N1 For) or CMV forward
  • 3′ sequencing primer TCCAGCTCGACCAGGATG (pEGFP-N1 Rev)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Template was MGC clone of I.M.A.G.E. consortium from the PlasmID Repository,
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1_hTRIM32 was a gift from Martin Dorf (Addgene plasmid # 69541 ; ; RRID:Addgene_69541)
  • For your References section:

    TRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymerase. Fu B, Wang L, Ding H, Schwamborn JC, Li S, Dorf ME. PLoS Pathog. 2015 Jun 9;11(6):e1004960. doi: 10.1371/journal.ppat.1004960. eCollection 2015 Jun. PPATHOGENS-D-15-00349 [pii] PubMed 26057645