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(Plasmid #50015)


Item Catalog # Description Quantity Price (USD)
Plasmid 50015 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Agilent (Stratagene)
  • Backbone size w/o insert (bp) 5191
  • Total vector size (bp) 8423
  • Modifications to backbone
    Replaced with new MCS: XhoI-HindIII-BamHI-NotI-Insert[(TGACTCCTGCCTCAGG(5'UT)-Gene CDS(no Stop)]-ClaI -3xFLAG
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    ring finger protein 31
  • Alt name
  • Alt name
  • Alt name
    E3 ubiquitin-protein ligase RNF31
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
    BC012077 NM_017999
  • Entrez Gene
    RNF31 (a.k.a. HOIP, Paul, ZIBRA)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3 x FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Open Biosystems / Thermo Scientific MHS1011-76068 Human MGC Verified FL cDNA (IRAU)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV3flag8HOIP was a gift from Martin Dorf (Addgene plasmid # 50015 ; ; RRID:Addgene_50015)
  • For your References section:

    The ubiquitin conjugating enzyme UBE2L3 regulates TNFalpha-induced linear ubiquitination. Fu B, Li S, Wang L, Berman MA, Dorf ME. Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133. 10.1038/cr.2013.133 PubMed 24060851