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(Plasmid #78118)


Item Catalog # Description Quantity Price (USD)
Plasmid 78118 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5397
  • Total vector size (bp) 6818
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Endoplasmic reticulum targeted GAP3
  • Species
  • Insert Size (bp)
  • Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D24N/D119A in aequorin moiety and
  • Promoter CMV
  • Tags / Fusion Proteins
    • calreticulin signal peptide (N terminal on backbone)
    • KDEL (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3_erGAP3 was a gift from Teresa Alonso & Javier García-Sancho (Addgene plasmid # 78118 ; ; RRID:Addgene_78118)
  • For your References section:

    GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Navas-Navarro P, Rojo-Ruiz J, Rodriguez-Prados M, Ganfornina MD, Looger LL, Alonso MT, Garcia-Sancho J. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. doi: 10.1016/j.chembiol.2016.05.010. 10.1016/j.chembiol.2016.05.010 PubMed 27291400