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pClneoMyc-LATS1
(Plasmid #66851)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66851 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pClneo
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LATS1
  • Alt name
    large tumor suppressor kinase 1
  • Species
    H. sapiens (human)
  • Mutation
    P44L mutation in LATS1 compared to GenBank reference sequence NP_004681.1
  • GenBank ID
    NM_004690.3
  • Entrez Gene
    LATS1 (a.k.a. WARTS, wts)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Addgene sequencing results find amino acid change P44L compared to available LATS1 reference sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoMyc-LATS1 was a gift from Yutaka Hata (Addgene plasmid # 66851 ; http://n2t.net/addgene:66851 ; RRID:Addgene_66851)
  • For your References section:

    A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, Okuno Y, Hata S, Nishina H, Hata Y. J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 2011 May 17. 10.1093/jb/mvr063 PubMed 21586534