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pCW57.1-DUX4-WT
(Plasmid #99282)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 99282 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCW57.1
  • Backbone size w/o insert (bp) 9354
  • Total vector size (bp) 9132
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    We used Stbl3 cells (recombination deficient)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DUX4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1275
  • Entrez Gene
    DUX4 (a.k.a. DUX4L)
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer tcgcctggagaattggc
  • 3′ sequencing primer agggctgccttggaaaag
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-DUX4-WT was a gift from Stephen Tapscott (Addgene plasmid # 99282 ; http://n2t.net/addgene:99282 ; RRID:Addgene_99282)
  • For your References section:

    Model systems of DUX4 expression recapitulate the transcriptional profile of FSHD cells. Jagannathan S, Shadle SC, Resnick R, Snider L, Tawil RN, van der Maarel SM, Bradley RK, Tapscott SJ. Hum Mol Genet. 2016 Oct 15;25(20):4419-4431. doi: 10.1093/hmg/ddw271. 10.1093/hmg/ddw271 PubMed 28171552