Skip to main content
Addgene
Open Menu

pGL3 Zscan4 promoter
(Plasmid #69249)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69249 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 69249-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 69249-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pGL3 basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5583
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zscan4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    765
  • Mutation
    The two single nucleotide mismatches found between the depositor's insert sequence and Addgene’s sequences have no functional consequences (C>G at bp 59 and A>G at bp 399).
  • Entrez Gene
    ZSCAN4 (a.k.a. ZNF494)
  • Promoter Zscan4

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer RVp3
  • 3′ sequencing primer GLp2
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The two single nucleotide mismatches found between the depositor's insert sequence and Addgene’s sequences have no functional consequences.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3 Zscan4 promoter was a gift from Stephen Tapscott (Addgene plasmid # 69249 ; http://n2t.net/addgene:69249 ; RRID:Addgene_69249)

  • For your References section:

    DUX4 activates germline genes, retroelements, and immune mediators: implications for facioscapulohumeral dystrophy. Geng LN, Yao Z, Snider L, Fong AP, Cech JN, Young JM, van der Maarel SM, Ruzzo WL, Gentleman RC, Tawil R, Tapscott SJ. Dev Cell. 2012 Jan 17;22(1):38-51. doi: 10.1016/j.devcel.2011.11.013. Epub 2011 Dec 29. 10.1016/j.devcel.2011.11.013 PubMed 22209328
Related items:
Commonly requested with: