pGL3 Zscan4 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69249||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL3 basic
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 5583
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)765
MutationThe two single nucleotide mismatches found between the depositor's insert sequence and Addgene’s sequences have no functional consequences (C>G at bp 59 and A>G at bp 399).
Entrez GeneZSCAN4 (a.k.a. ZNF494)
- Promoter Zscan4
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer RVp3
- 3′ sequencing primer GLp2 (Common Sequencing Primers)
related information can be found in:
DUX4 activates germline genes, retroelements, and immune mediators: implications for facioscapulohumeral dystrophy.
Geng LN, Yao Z, Snider L, Fong AP, Cech JN, Young JM, van der Maarel SM, Ruzzo WL, Gentleman RC, Tawil R, Tapscott SJ.
Dev Cell. 2012 Jan 17;22(1):38-51. doi: 10.1016/j.devcel.2011.11.013. Epub 2011 Dec 29.
PMID: 22209328. The two single nucleotide mismatches found between the depositor's insert sequence and Addgene’s sequences have no functional consequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3 Zscan4 promoter was a gift from Stephen Tapscott (Addgene plasmid # 69249 ; http://n2t.net/addgene:69249 ; RRID:Addgene_69249)