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Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.
East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA
Nature. 2016 Sep 26. doi: 10.1038/nature19802.
PubMed Article

Plasmids from Article

ID Plasmid  
83482p2CT-His-MBP-Lbu_C2c2_WT
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83483p2CT-His-MBP-Lbu_C2c2_R472A_H477A
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83484p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A
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83485p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
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83486p2CT-His-MBP-Lse_C2c2_WT
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83487p2CT-His-MBP-Lsh_C2c2_WT
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83494p2CT-His-MBP-Lbu_C2c2_R1079A
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