Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.
East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA
Nature. 2016 Sep 26. doi: 10.1038/nature19802.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
83482p2CT-His-MBP-Lbu_C2c2_WTBacterial expression of WT L. buccalis C2c2 CRISPR effector.
Loading...
83483p2CT-His-MBP-Lbu_C2c2_R472A_H477ABacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).
Loading...
83484p2CT-His-MBP-Lbu_C2c2_R1048A_H1053ABacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).
Loading...
83485p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053ABacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).
Loading...
83486p2CT-His-MBP-Lse_C2c2_WTBacterial expression of WT L. seeligeri C2c2 CRISPR effector
Loading...
83487p2CT-His-MBP-Lsh_C2c2_WTBacterial expression of WT L. shahii C2c2 CRISPR effector
Loading...
83494p2CT-His-MBP-Lbu_C2c2_R1079ABacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)
Loading...