PurposeBacterial expression of WT L. seeligeri C2c2 CRISPR effector
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83486||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5960
- Total vector size (bp) 9320
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Insert Size (bp)3360
/ Fusion Protein
- His6-MBP (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer MBP forward (Common Sequencing Primers)
Codon optimized for E. Coli expression. His-MBP fusion tag can be removed with TEV protease.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p2CT-His-MBP-Lse_C2c2_WT was a gift from Jennifer Doudna (Addgene plasmid # 83486 ; http://n2t.net/addgene:83486 ; RRID:Addgene_83486)
For your References section:Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA. Nature. 2016 Sep 26. doi: 10.1038/nature19802. 10.1038/nature19802 PubMed 27669025