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(Plasmid #83485)


Item Catalog # Description Quantity Price (USD)
Plasmid 83485 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5960
  • Total vector size (bp) 9437
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    Leptotrichia buccalis
  • Insert Size (bp)
  • Mutation
    R472A, H477A, R1048A, H1053A
  • GenBank ID
  • Tag / Fusion Protein
    • His6-MBP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Codon optimized for E. Coli expression. His-MBP fusion tag can be removed with TEV protease.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A was a gift from Jennifer Doudna (Addgene plasmid # 83485 ; ; RRID:Addgene_83485)
  • For your References section:

    Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA. Nature. 2016 Sep 26. doi: 10.1038/nature19802. 10.1038/nature19802 PubMed 27669025