163,606 results

1. Ube1/PET21d

   Plasmid

   #34965

   Depositor

   Cynthia Wolberger

   Article

   Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

   Insert

   Ube1 (UBA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

   Plasmid

   #217635

   Purpose

   AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

   Depositor

   Martin Kampmann

   Article

   Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

   Insert

   mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha and mU6

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-pCAG eGFP T2A SP HALO HAPLN1

   Plasmid

   #228200

   Purpose

   AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

   Depositor

   Sungjin Park

   Article

   Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

   Insert

   HAPLN1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   constitutive CAG promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB_TRE3G_scFV-sfGFP-Prdm9CD

   Plasmid

   #235577

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Prdm9 (Prdm9 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnCS_SEPT6_SEPT7-TEV-Strep

   Plasmid

   #174499

   Purpose

   bacterial co-expression of human SEPT6 and of human SEPT7

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN6 (SEPTIN6 Human)
   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG-EYFP

   Plasmid

   #34582

   Depositor

   Celeste Nelson

   Article

   Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

   Type

   Empty backbone

   Use

   Tags

   3xFLAG and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-Bmpr2

   Plasmid

   #163403

   Purpose

   Expresses BMPR-2 in mammalican cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW-FIRE-pHLy

   Plasmid

   #170774

   Purpose

   Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   hUbC

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.2-FUS-1-526aa-V5

    Plasmid

    #29609

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB414

    Plasmid

    #74808

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. scAAV-hIBA1a-GFP

    Plasmid

    #214146

    Purpose

    Self-complementary AAV vector packaging GFP under the human Iba1 promoter

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) CircRNA Mini Vector

    Plasmid

    #60648

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191796

    Purpose

    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13X
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R84A, H89A, R739A, R740A, H745A, H746A, H747A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-TetOne-zeo_3xHA-TurboID-NLS

    Plasmid

    #200022

    Purpose

    For lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoter

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    3xHA-TurboID-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3Gs

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB_TRE3G_scFV-sfGFP-Kmt5cFL

    Plasmid

    #235581

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pL6puro-JeT-pmRevErbA-mScaI3-NLS-Pest

    Plasmid

    #240107

    Purpose

    A lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    added JeT Promoter

    Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF.myc.ER-E2-Crimson

    Plasmid

    #38770

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Use

    Tags

    ER retention signal (KDEL), ER signal sequence (M…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PEA1-GFP

    Plasmid

    #171993

    Purpose

    Delivers all prime editing nickase components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge