173,051 results

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dCas9-hHDAC3

Plasmid

#98591

Purpose

3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCAS9-HDAC3 (HDAC3 S. pyogenes, Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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SIRT5 Flag

Plasmid

#13816

Purpose

Mammalian expression of human SIRT5 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT5 (SIRT5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pEG BacMam C term 3C eGFP His8

Plasmid

#160687

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP His8

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCE-hUL

Plasmid

#41855

Purpose

Non-integrating (episomal) expression of human L-MYC and LIN28

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

L-MYC, LIN28 (MYCL, LIN28A Human)

Expression

Mammalian

Promoter

CAG

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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PGK-AAVS1ZFNR

Plasmid

#60915

Purpose

encodes zinc finger nuclease specific to AAVS1 locus (right)

Depositor

Paul Gadue

Article

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

AAVS1-ZFNR

Promoter

PGK

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

Plasmid

#225754

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2

Plasmid

#68161

Purpose

Universal domesticator plasmid for level 0 parts

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pnCasPA-BEC

Plasmid

#113349

Purpose

A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N106

Plasmid

#66808

Purpose

2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-HRD1

Plasmid

#231688

Purpose

Expresses human HRD1 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

HRD1 (SYVN1 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2_HA_dreAdar1

Plasmid

#250540

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

Plasmid

#67981

Purpose

Cas9 activity reporter (control) with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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p236

Plasmid

#186182

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 236

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi

Plasmid

#53277

Purpose

Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

idi

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoter

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 TBRI-His

Plasmid

#19161

Depositor

Joan Massague

Insert

transforming growth factor, beta receptor 1 (TGFBR1 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCE-hSK

Plasmid

#41814

Purpose

Non-integrating (episomal) expression of human SOX2 and KLF4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

SOX2, KLF4 (SOX2, KLF4 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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