Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration.
Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T
Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. PubMed Article
Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T
Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155217 | C1-F-tractin-mCitrine | Monitor global F-actin distribution |
| 155218 | C1-F-tractin-mCherry | Monitor global F-actin distribution |
| 155219 | C1-F-tractin(delta1-6)-mCitrine | F-actin bidning mutant of F-tractin |
| 155220 | C1-MPAct-mCitrine | Monitor changes in the density of membrane-proximal F-actin (MPA) |
| 155221 | C1-MPAct-mRuby3 | Monitor changes in the density of membrane-proximal F-actin (MPA) |
| 155222 | C1-MPAct-mCherry | Monitor changes in the density of membrane-proximal F-actin (MPA) |
| 155223 | C1-MPAct(delta1-6)-mRuby3 | F-actin bidning mutant of MPAct |
| 155224 | C1-FT-mCitrine-EAAARx8-CaaX | MPAct with increased search radius |
| 155225 | C1-FT-mCitrine-EEVEE-CaaX | MPAct with increased linker flexiblity |
| 155226 | C1-Lyn-mCitrine-EEVEE-LifeAct | Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin |
| 155227 | C1-mCitrine-FKBP-EZRabd(t567D) | Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin |
| 155228 | pCAG-Lyn11-FRB | membrane anchor for FKBP-FRB system |
| 155229 | C1-iRFP-iLid-CaaX | membrane marker |
| 155230 | pLV-MPAct-mCit-IRES-Neo | lentiviral MPAct construct |
| 155231 | pLV-MPAct-mRuby3-IRES-Puro | lentiviral MPAct construct |
| 155232 | CFP-CaaX | membane marker |
| 155233 | YFP-CaaX | membane marker |
| 155234 | C1-CFP-Lyn11 | membane marker |