pET-Sfp
(Plasmid #159617)

• Purpose: Expression plasmid for Sfp phosphopantetheinyl transferase
• Depositing Labs: Christopher T Walsh, Jun Yin
• Publication: Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46. doi: 10.1021/cb700054k. Epub 2007 Apr 27.

Backbone

• Vector backbone: pET
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sfp
• Species: Bacillus subtilis
• Insert Size (bp): 699
• Tag / Fusion Protein:
  • 6HIS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been found to be somewhat prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.