163,606 results

1. MAVS-WT

   Plasmid

   #52135

   Purpose

   Retroviral vector for expression of human MAVS in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

   Insert

   MAVS (MAVS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AIMTOR T757

   Plasmid

   #140828

   Purpose

   AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR T757 (ULK1 Human)
   

   Use

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Promoter

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DD-Cas9 with filler sequence and Venus (EDCPV)

   Plasmid

   #90085

   Purpose

   This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-EGFP-IRES-puro

   Plasmid

   #128652

   Purpose

   mammalian expression of EGFP with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ancBE4-NG

   Plasmid

   #226853

   Purpose

   Expresses ancBE4max-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ancBE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. NeonGreen-C1-ORP3

   Plasmid

   #220070

   Purpose

   overexpresses ORP3 with a neonGreen Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL3 (OSBPL3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHT-ads

   Plasmid

   #47382

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available Since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-BLAST flag-CAD

   Plasmid

   #169818

   Purpose

   Expresses C-terminal flag-tagged CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCCC -> AGTC silent mutations at nt527-530 eli…

   Promoter

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-sh1368 knockdown c-myc

   Plasmid

   #29435

   Depositor

   Bob Eisenman

   Article

   Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

   Insert

   c-Myc shRNA (MYC Human)
   

   Use

   Lentiviral and RNAi

   Tags

   GFP and WPRE

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-Cas9-Act3.0

    Plasmid

    #178954

    Purpose

    It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-mTurquoise-P2A-iCre (JDW 920)

    Plasmid

    #224518

    Purpose

    A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mTurquoise-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCpfSA

    Plasmid

    #187890

    Purpose

    CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

    Depositor

    Quanjiang Ji

    Article

    Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Use

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN3-Nipbl

    Plasmid

    #107716

    Purpose

    Expression plasmid for 3xFLAG-tagged NIPBL

    Depositor

    Guntram Suske

    Insert

    NIPBL (Nipbl Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pKM-U6-reRNA-acceptor-2xBbsI

    Plasmid

    #205633

    Purpose

    Cloning and U6-drive expression of ISDra2 TnpB reRNAs

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 VDAC1-Linker-GFP

    Plasmid

    #211734

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICSL90002

    Plasmid

    #68261

    Purpose

    Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6-26 (Arabidopsis thaliana)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Stephen Quake, Paul Blainey

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPROEX Aqua

    Plasmid

    #42889

    Depositor

    Fabienne Merola

    Article

    Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

    Insert

    Aquamarine
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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