161,709 results

1. pmtb-t7-alpha-bungarotoxin

   Plasmid

   #69542

   Purpose

   For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

   Depositor

   Sean Megason

   Article

   Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

   Insert

   alpha-bungarotoxin
   

   Use

   Tol2, beta-actin promoter, sp6,t7 for zebrafish

   Tags

   Expression

   Mutation

   Promoter

   beta-actin, sp6, t7

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-CADPS KI

   Plasmid

   #131482

   Purpose

   Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cadps Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-SEP GluR2(R)

   Plasmid

   #24001

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR2 (Gria2 Rat)
   

   Use

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB2F

   Plasmid

   #195289

   Purpose

   Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ1371-sgSTAT3-1

   Plasmid

   #121425

   Purpose

   sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgSTAT3-1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGF1-4eCOL2A1

   Plasmid

   #97210

   Purpose

   Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   COL2A1 regulatory elements

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_PIK3CA_p.H1047R

   Plasmid

   #82824

   Purpose

   Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   H1047R

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF6.mCherry-TSG101

   Plasmid

   #38318

   Depositor

   Quan Lu

   Article

   Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   TSG101 (TSG101 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet1_CoiA1_CoiB

    Plasmid

    #208761

    Purpose

    Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiA1
    CoiB
    

    Use

    Tags

    Hisx6 Tag and SUMO

    Expression

    Bacterial

    Mutation

    Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

    Promoter

    T7

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH6HTC_CCT2-Halo

    Plasmid

    #175337

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    CCT2 (CCT2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170995

    Purpose

    HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-GFP(S65T)-kanMX6

    Plasmid

    #39292

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCP60 RS-GFP

    Plasmid

    #122173

    Purpose

    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"

    Depositor

    Lukas Fischer

    Article

    Nocarova et al BMC Plant Biol. 2009 Apr 22;9:44. doi: 1

    Insert

    RS-GFP
    

    Use

    Binary vector for escherichia coli and agrobacter…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-PDK3

    Plasmid

    #23556

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDK3 (PDK3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pFA6a-link-yemiRFP670-Kan

    Plasmid

    #224088

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selection

    Depositor

    Bas Teusink

    Insert

    yemiRFP670
    

    Use

    Tags

    yemiRFP670

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-L4R1-hEF1a

    Plasmid

    #139523

    Purpose

    Provides constitutive promoter cassette between attL4-attR1 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    hEF1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF1337

    Plasmid

    #144171

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB7A (ZBTB7A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP_TOMM20_sfCherry2(11)

    Plasmid

    #83033

    Purpose

    Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    TOMM20_sfCherry+11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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