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pcDNA5/FRT/TO_NES_HaloTag7

Plasmid

#175527

Purpose

Strict cytosolic expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

NES-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Tags

GFP and Halotag

Expression

Mammalian

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRPF1

Plasmid

#65382

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF1 (BRPF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pH-EF1α-DualPE

Plasmid

#236417

Purpose

For plant prime editing including large DNA fragment editing in Solanum lycopersicum or other dicotyledons

Depositor

Zong Yuan

Article

Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:

Insert

EF1α promoter

Use

CRISPR

Expression

Plant

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GFP-Fishell-1

Plasmid

#83900

Purpose

GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

eGFP

Use

AAV

Promoter

mDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/SV-Venus-SIINFEKL

Plasmid

#111624

Purpose

Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

Depositor

Jon Yewdell

Insert

Venus-LEQLE-SIINFEKL-TEW

Tags

LEQLE-SIINFEKL-TEW

Expression

Mammalian

Promoter

SV40

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7

Plasmid

#64953

Purpose

mini-Tn7 base vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR4_G3BP2

Plasmid

#127105

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP2 (G3BP2 Human)

Use

Gateway Cloning

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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GIPR-DuET

Plasmid

#213252

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GIPR (GIPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

human fission factor1 (FIS1 Human)

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAF0687 Pa01_attP-mCherry donor

Plasmid

#193466

Purpose

Promoterless mcherry donor for Pa01

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag7

Plasmid

#175533

Purpose

Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#28014

Depositor

Karel Svoboda

Article

Mao et al Neuron. 2011 Oct 6;72(1):111-23.

Insert

eGFP

Use

AAV; Adeno associate viral

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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AAV GFP

Plasmid

#49055

Purpose

adeno-associated virus (AAV) encoding a green fluorescent protein

Depositor

Fred Gage

Article

Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCRY2PHR-mCherryN1

Plasmid

#26866

Purpose

Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2PHR (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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