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TurboID-V5_pRS415

Plasmid

#107167

Purpose

expresses V5-tagged TurboID in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

Expression

Yeast

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pT2KXIG (Tol2)-tp63:ikk1:GFP

Plasmid

#105646

Purpose

Ikk1(alpha) expression in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1:GFP (chuk Zebrafish)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein

Plasmid

#117848

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D770-N771 insNPG

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Use

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

Plasmid

#224123

Purpose

Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-mutant (Q20A_Y23A_Y24F_F35A)

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Q20A_Y23A_Y24F_F35A in La(SSB)1-194

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-cdc42palm

Plasmid

#20141

Depositor

Alaa El-Husseini

Article

Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

Insert

cell division cycle 42 isoform 2 (CDC42 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

wild type

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
Ube1/PET21d

Plasmid

#34965

Depositor

Cynthia Wolberger

Article

Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

Insert

Ube1 (UBA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Delta [N151/3.3R]

Plasmid

#114515

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Delta (Rat). Derived from hybridoma N151/3.3.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GABA(A)R, Delta (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabrd Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas12a(RuvC)

Plasmid

#186447

Purpose

Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12a

Use

CRISPR

Tags

Expression

Mutation

D917A, E1006A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha4 [N398A/34R]

Plasmid

#177543

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha4 (Rat). Derived from hybridoma N398A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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PB_EF1a-DjCas13d-msfGFP-Blast

Plasmid

#226008

Purpose

DjCas13d protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

DjCas13d RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHCT2

Plasmid

#130970

Purpose

HyPr plasmid with natMX marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHMK34

Plasmid

#130971

Purpose

HyPr plasmid with hphMX marker

Depositor

Chris Todd Hittinger

Article

Peris et al Nat Commun. 2020 Apr 29;11(1):2085. doi:

Insert

empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Derived from pGL4.23

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR2c-V5/HIS

Plasmid

#201107

Purpose

expression of human FGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)

Use

Tags

V5/His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-cdc42prenyl

Plasmid

#20142

Depositor

Alaa El-Husseini

Article

Kang et al Nature. 2008 Dec 18. 456(7224):904-9.

Insert

cell division cycle 42 isoform 1 (CDC42 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-GFP-IKBalpha-mut (super repressor)

Plasmid

#15264

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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