163,606 results

1. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

   Plasmid

   #106171

   Purpose

   cre mediated red to green shift

   Depositor

   Ewa Snaar-Jagalska

   Article

   Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

   Inserts

   dsRED-express2
   eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-MBP-BrCas12b-RFND

   Plasmid

   #195339

   Purpose

   Expresses Engineered BrCas12b in Bacteria

   Depositor

   Piyush Jain

   Article

   Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis and 6xHis, MBP, TEV cleavage site

   Expression

   Bacterial

   Mutation

   R160E, F208W, N524V, D868V

   Promoter

   T7

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LBJJ383

   Plasmid

   #117517

   Purpose

   PCR template for sgRNA classic with polyT as terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_iMYOD1_P2A_GFP_Puro

   Plasmid

   #168804

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-3-FT-ENGRAM

   Plasmid

   #179158

   Purpose

   piggyBac plasmid for 3'-FT ENGRAM recorder

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Insert

   Csy4
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minP

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. etnppl-pGEMtEasy

   Plasmid

   #192263

   Purpose

   Mouse Etnppl sequence is inserted in pGEM-T Easy vector.

   Depositor

   Toshihide Yamashita

   Article

   Tsujioka et al Front Cell Neurosci. 2023 Jan 30;17:1097

   Insert

   ethanolamine phosphate phospholyase (Etnppl Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Promoter

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-NPM WT

   Plasmid

   #17578

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Use

   Tags

   EGFP and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest eGFP MBNL1 42 kDa

   Plasmid

   #61277

   Purpose

   Mammalian expression of EGFP-tagged human MBNL1

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   MBNL1 (MBNL1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF-G1(6/7FTrp)RS

    Plasmid

    #207621

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-47: CTNNB1-mEGFP

    Plasmid

    #109119

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Beta1-GFP in pHcgreen

    Plasmid

    #69804

    Purpose

    Expresses Beta1-GFP integrin for normal cell transfection

    Depositor

    Martin Humphries

    Article

    Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

    Insert

    Beta1A integrin (ITGB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG-FR-GECO1a

    Plasmid

    #163681

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1a

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p6sheLLr

    Plasmid

    #37318

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

    Insert

    HPV6L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CMV-SauriCas9

    Plasmid

    #135964

    Purpose

    Expresses SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge