The pST44 polycistronic expression system for producing protein complexes in Escherichia coli.
Tan S, Kern RC, Selleck W
Protein Expr Purif. 2005 Apr;40(2):385-95. PubMed Article
Tan S, Kern RC, Selleck W
Protein Expr Purif. 2005 Apr;40(2):385-95. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63935 | pST50Trc1-HISDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63936 | pST50Trc1-CBPDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63937 | pST50Trc1-STRDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63938 | pST50Trc1-FLAGDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63939 | pST50Trc1-HPCDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63940 | pST50Trc1-HISNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63941 | pST50Trc1-CBPNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63942 | pST50Trc1-STRNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63943 | pST50Trc1-FLAGNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63944 | pST50Trc1-HPCNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63945 | pST50Trc1-CBPHISNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63946 | pST50Trc1-STRHISNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63947 | pST50Trc1-FLAGHISNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63948 | pST50Trc1-HPCHISNDHFR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63949 | pST50Trc1-DHFRHIS | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63950 | pST50Trc1-DHFRSTR | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63951 | pST50Trc1-DHFRFLAG | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63952 | pST50Trc1-DHFRCYD | Position 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63953 | pST50Trc2-HISDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63954 | pST50Trc2-CBPDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63955 | pST50Trc2-STRDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63956 | pST50Trc2-FLAGDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63957 | pST50Trc2-HPCDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63958 | pST50Trc2-HISNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63959 | pST50Trc2-CBPNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63960 | pST50Trc2-STRNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63961 | pST50Trc2-FLAGNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63962 | pST50Trc2-HPCNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63963 | pST50Trc2-CBPHISNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63964 | pST50Trc2-STRHISNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63965 | pST50Trc2-FLAGHISNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63966 | pST50Trc2-HPCHISNDHFR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63967 | pST50Trc2-DHFRHIS | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63968 | pST50Trc2-DHFRSTR | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63969 | pST50Trc2-DHFRFLAG | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63970 | pST50Trc2-DHFRCYD | Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63971 | pST50Trc3-HISDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63972 | pST50Trc3-CBPDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63973 | pST50Trc3-STRDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63974 | pST50Trc3-FLAGDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63975 | pST50Trc3-HPCDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63976 | pST50Trc3-HISNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63977 | pST50Trc3-CBPNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63978 | pST50Trc3-STRNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63979 | pST50Trc3-FLAGNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63980 | pST50Trc3-HPCNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63981 | pST50Trc3-CBPHISNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63982 | pST50Trc3-STRHISNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63983 | pST50Trc3-FLAGHISNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63984 | pST50Trc3-HPCHISNDHFR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63985 | pST50Trc3-DHFRHIS | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63986 | pST50Trc3-DHFRSTR | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63987 | pST50Trc3-DHFRFLAG | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63988 | pST50Trc3-DHFRCYD | Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63989 | pST50Trc4-HISDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63990 | pST50Trc4-CBPDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63991 | pST50Trc4-STRDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63992 | pST50Trc4-FLAGDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63993 | pST50Trc4-HPCDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63994 | pST50Trc4-HISNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63995 | pST50Trc4-CBPNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63996 | pST50Trc4-STRNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63997 | pST50Trc4-FLAGNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63998 | pST50Trc4-HPCNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 63999 | pST50Trc4-CBPHISNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64000 | pST50Trc4-STRHISNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64001 | pST50Trc4-FLAGHISNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64002 | pST50Trc4-HPCHISNDHFR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64003 | pST50Trc4-DHFRHIS | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64004 | pST50Trc4-DHFRSTR | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64005 | pST50Trc4-DHFRCYD | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64006 | pST50Trc4-DHFRFLAG | Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control |
| 64007 | pST44 | polycistronic cloning vector for pST44 plasmid suite |
| 64008 | pET3aTr | Single donor or transfer plasmid for source of translation cassette for plasmid suite |
| 64009 | pST39 | original polycistronic cloning vector for plasmid suite (generation I) |