pUltra-puro-RTTA3
(Plasmid #58750)

• Purpose: silence doxycyclin inducible pUltra-dox
• Depositing Labs: Yildirim Dogan, Kitai Kim
• Publication: pUltra plasmids (unpublished)

Backbone

• Vector backbone: pUltra-Chili
• Backbone manufacturer: Moore Lab, Addgene plasmid 48687
• Modifications to backbone: dtomato was replaced with puro
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Reverse TET-Transactivator 3
• Alt name: RTTA3
• Mutation: loxP5171
• Promoter: Ubiquitin C promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: hUBCpro-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RTTA3 was amplified from pSLIK-Neo-TGmiR-luc (Addgene plasmid 25743).

loxP5171 at positions after cppt and before WPRE