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pUltra-dox
(Plasmid #58749)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58749 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUltra-Chili
  • Backbone manufacturer
    Moore Lab, Addgene plasmid 48687
  • Modifications to backbone
    replace Ubic-promoter in pUltra-chili with Tre2. Tre2 was amplified from pSLIK-Neo-TGmiR-luc (Addgene plasmid 25745).
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Promoter TRE2 upstream of minimal CMV promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid needs to be used with a second plasmid containing a constitutively expressed puromycin-selectable RTTA3 (Reverse Tet-Transactivator)--Addgene plasmids 58750 and 58751.

The advantage to other dox-inducible systems are:
1) better silencing in off condition because of the newer generation TRE2/RTTA3
2) you can induce to express two genes of interest (GOI) at the same time from one plasmid.
3) when the system is induced by dox, besides the GOI the tomato-reporter is induced to be expressed too, so you can actually see that the genes must be switched on.

loxP5171 at positions after cppt and before WPRE

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltra-dox was a gift from Yildirim Dogan & Kitai Kim (Addgene plasmid # 58749 ; http://n2t.net/addgene:58749 ; RRID:Addgene_58749)