165,971 results

1. pDONR221_EGFP

   Plasmid

   #25899

   Purpose

   Gateway entry vector containing EGFP.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-Calreticulin-N-16

   Plasmid

   #54023

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Calreticulin (CALR Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHcRed-NPM1c-C1

   Plasmid

   #131821

   Purpose

   Expresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Tags

   HcRed

   Expression

   Mammalian

   Mutation

   This is the cytoplasmic mutant causing AML, where…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

   Plasmid

   #162377

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rtn4a-GFP

   Plasmid

   #61807

   Purpose

   Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

   Insert

   Rtn4a (RTN4 Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Rtn4a Stop Codon removed

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-polyQ74-NLS

   Plasmid

   #231892

   Purpose

   Forms nuclear HTT partial exon 1 Q74-NLS aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. G555

   Plasmid

   #216483

   Purpose

   Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

   Depositor

   Christophe Danelon

   Insert

   plsB, plsC, cdsA, pssA
   

   Use

   Cell-free expression

   Promoter

   T7 for each gene

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-3'3xFlag

    Plasmid

    #208616

    Purpose

    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP Cortactin

    Plasmid

    #50728

    Purpose

    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cortactin (CTTN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C3-ZO-2

    Plasmid

    #27422

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

13. His-GFP-Rab27a

    Plasmid

    #170861

    Purpose

    Mammalian expression of His-GFP-Rab27a.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Rab-27a (RAB27A Human)
    

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-HaloTag-KanMX6

    Plasmid

    #87029

    Purpose

    Tag S. pombe genes in C terminal with HaloTag

    Depositor

    Julien Berro

    Insert

    HaloTag
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-KG-GST-YAP

    Plasmid

    #33052

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIB2

    Plasmid

    #25451

    Depositor

    Benjamin Glick

    Article

    Sears et al Yeast. 1998 Jun 15;14(8):783-90.

    Insert

    Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
    

    Use

    Pichia pastoris expression

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. R777-E172 Hs.PIK3R1-nostop

    Plasmid

    #70456

    Purpose

    Gateway ORF clone of human PIK3R1 [NM_181523.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. KRAB-dCas9

    Plasmid

    #112195

    Purpose

    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    KRAB (ZNF10 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-KRAB-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS316-RGR-GFP

    Plasmid

    #51056

    Purpose

    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINDUCER20_3xFlag

    Plasmid

    #190028

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlag

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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