179,844 results
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Plasmid#167577PurposeExpress the ATP sensor GRAB_ATP1.0 in neuronsDepositorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159816PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE
Plasmid#162382PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmem-TurboID
TagsHA and V5ExpressionMammalianPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JD553
Plasmid#160392PurposeWheat GRF4-GIF1 chimera in pDONR-zeoDepositorInsertwheat GRF4-GIF1 chimera
UseEntry clonAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorExpressionMammalianPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRPromoterEFSAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-Syn-PSD95.FingR-eGFP-CCR5TC
Plasmid#125693PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MLRV2: NF-kb-SMAD-DBE-P53-AP1
Plasmid#124536PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserts5 copies of the NF-kb DNA binding motif
7xSMAD_RE::FLuc
3xDBE_BE::Renilla (FOXO)
2xP53_RE::NLuc
6xAP-1_RE::GrRenilla
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shNRF2 #1
Plasmid#136584PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX165-HiFi Cas9
Plasmid#140563PurposeExpresses HiFi Cas9 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
MutationR691AAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 ORF7B
Plasmid#141277PurposeGateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCEP4-myc-HLA-A2
Plasmid#135504PurposeMammalian expression of myc-tagged HLA-A*02:01DepositorInsertHLA-A*02:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Sport6-CD63-pHuji
Plasmid#130902PurposeExpression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD63-pHuji (CD63 Human, synthetic)
TagspHujiExpressionMammalianMutationpHuji inserted between Gln-36 and Leu-37 in extra…PromoterCMVAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HR180-LGR5-iCT
Plasmid#129094PurposeKI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130999PurposeDouble floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoterDepositorInsertChRmine
UseAAVTagsmScarlet-Kv2.1PromoterEf1aAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by