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pGR481

Plasmid

#213783

Purpose

Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

Depositor

Chang Liu

Insert

ura3*

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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cyto-iATPSnFR1.0

Plasmid

#102550

Purpose

cytoplasmic ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-NPM1c-IRES-mCherry

Plasmid

#131825

Purpose

Lentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transduction

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDB5050

Plasmid

#171126

Purpose

A plasmid containing the codon-optimized OsTIR1, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

Insert

OsTIR1

Use

Other

Tags

Expression

Mutation

non applicable

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-IRES-puro

Plasmid

#128652

Purpose

mammalian expression of EGFP with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

EGFP

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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SigmaR1-mNeonGreen

Plasmid

#226566

Purpose

Encodes SigmaR1 protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti CMVTRE3G eGFP Puro (w819-1)

Plasmid

#27570

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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Galt1-Grx1-roGFP1-iE

Plasmid

#230029

Purpose

Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (mCherry)

Plasmid

#102945

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only
GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Article

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-crRNA

Plasmid

#194967

Purpose

The plasmid expresses Cas12j-8-crRNA expression elements.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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SV-ABAactDA

Plasmid

#38247

Depositor

Jerry Crabtree

Article

Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

Inserts

VP16/PYL1
Gal4DBD/ABI*

Use

Tags

Flag, Gal4DBD, HA, and VP16

Expression

Mammalian

Mutation

D143A

Promoter

SV40 (IRES) and SV40p

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-HA-UCHL5

Plasmid

#22565

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL5 (UCHL5 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-V0A

Plasmid

#210347

Purpose

Expresses ATP6V0A in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

V-ATPase V0A (ATP6V0A1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHREAC

Plasmid

#134908

Purpose

Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

double 35S

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-p53

Plasmid

#12091

Depositor

Tyler Jacks

Article

Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

Insert

p53 (TP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

72R variant of p53

Promoter

Available since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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