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Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection.
McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 10.1073/pnas.1915562116. Epub 2019 Dec 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
135485pCEP4-myc-HLA-A2-VNMammalian expression of VN-fused and myc-tagged HLA-A*02:01
135486pCEP4-myc-HLA-A2-VN (I52P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (I52P mutant)
135492pCEP4-myc-HLA-A2-VN (Y59P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y59P mutant)
135493pCEP4-myc-HLA-A2-VN (D61P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (D61P mutant)
135494pCEP4-myc-HLA-A2-VN (V67P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (V67P mutant)
135495pCEP4-myc-HLA-A2-VN (Y84P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)
135496pCEP4-myc-HLA-A2-VN (G112P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (G112P mutant)
135497pCEP4-myc-HLA-A2-VN (L156P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (L156P mutant)
135498pCEP4-myc-HLA-A2-VN (E166P)Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)
135499pCEP4-FLAG-TAPBPR-TM-VCMammalian expression of VC-fused and FLAG-tagged TAPBPR with MHC TM
135500pCEP4-FLAG-TAPBPRMammalian expression of FLAG-tagged TAPBPR
135501pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)Mammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)
135502pCEP4-myc-HLA-A1-VNMammalian expression of VN-fused and myc-tagged HLA-A*01:01
135503pCEP4-myc-HLA-A1Mammalian expression of myc-tagged HLA-A*01:01
135504pCEP4-myc-HLA-A2Mammalian expression of myc-tagged HLA-A*02:01
135505pCEP4-myc-HLA-A3Mammalian expression of myc-tagged HLA-A*03:01
135506pCEP4-myc-HLA-A3-VNMammalian expression of VN-fused and myc-tagged HLA-A*03:01
135507pCEP4-myc-HLA-A24Mammalian expression of myc-tagged HLA-A*024:02
135508pCEP4-myc-HLA-A24-VNMammalian expression of VN-fused and myc-tagged HLA-A*24:02
135509pCEP4-myc-HLA-B7Mammalian expression of myc-tagged HLA-B*07:02
135511pCEP4-myc-HLA-B8-VNMammalian expression of VN-fused and myc-tagged HLA-B*08:01
135512pCEP4-myc-HLA-B8Mammalian expression of myc-tagged HLA-B*08:01
135513pCEP4-myc-HLA-B15Mammalian expression of myc-tagged HLA-B*15:01
135514pCEP4-myc-HLA-B15-VNMammalian expression of VN-fused and myc-tagged HLA-B*15:01
135515pCEP4-myc-HLA-B57-VNMammalian expression of VN-fused and myc-tagged HLA-B*57:01
135516pCEP4-myc-HLA-B57Mammalian expression of myc-tagged HLA-B*57:01
135517pCEP4-myc-H2-Dd-VNMammalian expression of VN-fused and myc-tagged H2-Dd
135518pCEP4-myc-H2-DdMammalian expression of myc-tagged H2-Dd
135519pCEP4-myc-H2-LdMammalian expression of myc-tagged H2-Ld
135520pCEP4-myc-H2-Ld-VNMammalian expression of VN-fused and myc-tagged H2-Ld
136063pCEP4-myc-H2-Dd (Y84C/C121S/A139C)Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Antibodies from Article