Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways.
Sarrion-Perdigones A, Gonzalez Y, Venken KJT
Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. PubMed Article
Sarrion-Perdigones A, Gonzalez Y, Venken KJT
Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124524 | AlphaB | pColE1_AlphaB |
| 124525 | AlphaC | pColE1_AlphaC |
| 124526 | AlphaD | pColE1_AlphaD |
| 124527 | AlphaE | pColE1_AlphaE |
| 124528 | Omega Destination-CMV::ELuc:bGH | Omega destination vector, that has a CMV:ELUC:bgH in the backbone |
| 124529 | TB:3xDBE_RE:MiniP | 3 Copies of the Daf-16 family binding element |
| 124530 | 5xNF-kb_RE::RedF | NFKB RedFirefly luciferase reporter |
| 124531 | 7xSMAD_RE::FLuc | SMAD2 FLuc luciferase reporter |
| 124532 | 7xE-Box::Renilla | c-Myc Renilla luciferase reporter |
| 124533 | 2xP53_RE::NLuc | p53 NLuc luciferase reporter |
| 124534 | 6xAP-1_RE::GrRenilla | AP-1 GreenRenilla luciferase reporter |
| 124535 | 3xDBE_RE::Renilla | Foxo Renilla luciferase reporter |
| 124536 | MLRV2: NF-kb-SMAD-DBE-P53-AP1 | Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1 |
| 133878 | TB:5xNF- κβ_RE:MiniP | 5 copies of the NF-κβ DNA binding motif |
| 133879 | TB:7xSMAD_RE:MiniP | 7 copies the SMAD DNA binding motif |
| 133880 | TB:7xE-Box:MiniP | 7 copies of the E-box motif |
| 133881 | TB:2xP53_RE:MiniP | 2 copies of the p53 DNA binding motif |
| 133884 | TB:6xAP-1_RE:MiniP | 6 copies of the AP-1 binding motif |