7xSMAD_RE::FLuc
(Plasmid #124531)

• Purpose: SMAD2 FLuc luciferase reporter
• Depositing Lab: Koen Venken
• Publication: Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.

Backbone

• Vector backbone: AlphaB Vector
• Backbone manufacturer: Venken Lab, #124524
• Backbone size w/o insert (bp): 2517
• Total vector size (bp): 4780
• Vector type: Mammalian Expression, Luciferase, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAD FLuc reporter
• Alt name: Transcription Blocker + 7 copies of the SMAD DNA binding motif + miniP, Firefly luciferase
• Species: H. sapiens (human), Synthetic

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: aat gag ctg att taa caa aaa ttt aac gcg
• 3′ sequencing primer: ctt ttt acg gtt cct ggc ctt ttg

Resource Information

• Supplemental Documents:
  • AB 7xSMAD_Firefly_bgHpolyA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert can be released with BsmBI for further cloning into Omega level vectors.