165,971 results

1. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   deletion of AA 1 to 45

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist GFP-Linker-VDAC1

   Plasmid

   #211735

   Purpose

   Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

   Depositor

   Roland Malli

   Article

   Pilic et al bioRxiv 2023.09.08.556841

   Insert

   Voltage-dependent anion channel 1 (VDAC1 )
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCk3

   Plasmid

   #136697

   Purpose

   Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-mKOR

   Plasmid

   #179285

   Purpose

   Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

   Depositor

   Christian Gruber

   Article

   Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

   Insert

   kappa opioid receptor (Oprk1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-RB1

   Plasmid

   #87836

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Insert

   sgRB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Expression

   Mammalian

   Promoter

   Tight TRE promoter and U6

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ECFP-Nucleus-7

   Plasmid

   #55346

   Purpose

   Localization: SV40 NLS Triplet, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Insert

   SV40 T-Antigen Nuclear Localization Signal (NLS)
   

   Tags

   ECFP-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. mCherry-XRCC1

   Plasmid

   #215142

   Purpose

   Expresses mCherry-XRCC1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   XRCC1 (XRCC1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV4-Tax WT

   Plasmid

   #23284

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   Tax
   

   Expression

   Mammalian

   Available Since

   April 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE8e-NG

   Plasmid

   #226856

   Purpose

   Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8e-NG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Shank2-HA /pCDNA3

    Plasmid

    #197334

    Purpose

    Plasmid expressing an antigen targeting Shank2

    Depositor

    Morgan Sheng

    Insert

    Shank2 (Shank2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid

    #117699

    Purpose

    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    

    Tags

    HA tag, Myc-NES, and NLS

    Expression

    Mammalian

    Mutation

    codon optimized for murine expression

    Promoter

    None, 2A peptide from porcine teschovirus-1 polyp…

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Ccdc134-IRES-BFP

    Plasmid

    #117266

    Purpose

    Retroviral overexpression of Ccdc134

    Depositor

    Sarah Teichmann

    Article

    Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

    Insert

    Ccdc134 (Ccdc134 )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-LYC

    Plasmid

    #53270

    Purpose

    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

    Insert

    crtE, crtI, crtB
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCk2

    Plasmid

    #136696

    Purpose

    Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

    Depositor

    Nicola Patron

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA

    Plasmid

    #220717

    Purpose

    AiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNIC-HIS6-NUSA-TEV-GG

    Plasmid

    #215798

    Purpose

    Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pcDNA4-TO-Puromycin-mVenus-MAP

    Plasmid

    #44118

    Depositor

    Dannel McCollum

    Article

    Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

    Type

    Empty backbone

    Tags

    mVenus-MAP

    Expression

    Mammalian

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. slc1a3b:myrGFP-P2A-H2AmCherry

    Plasmid

    #170208

    Purpose

    Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

    Depositor

    Kelly Monk

    Article

    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

    Insert

    slc1a3b (slc1a3b Zebrafish)
    

    Use

    Zebrafish

    Tags

    myrGFP-P2A-H2AmCherry

    Promoter

    slc1a3b

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-UCOE-SFFV-EGFP

    Plasmid

    #188900

    Purpose

    Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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