163,606 results

1. pGL2- Empty control -SV40-Luc

   Plasmid

   #26280

   Purpose

   Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. modRNAc0-ABE8e-P2A-GFP

   Plasmid

   #178177

   Purpose

   IVT template plasmid for making ABE8e-P2A-GFP modRNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   ABE8e
   

   Use

   Ivt template plasmid

   Tags

   P2A-GFP

   Expression

   Mutation

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1 hPGK-PuroR-pA donor

   Plasmid

   #22072

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PGK-Puro (PGK1 Human)
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. p31sheLL

   Plasmid

   #37322

   Depositor

   John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   HPV31L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

   Plasmid

   #60629

   Purpose

   Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 human MyoD (MYOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIV_Rev

   Plasmid

   #236244

   Purpose

   3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   SIV Rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-APEX2-C Cloning Vector

   Plasmid

   #128146

   Purpose

   To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Flag-APEX2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-RFP-P2A-MCS

   Plasmid

   #78933

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28 MBP_PrA/G-H6

   Plasmid

   #176077

   Purpose

   Express His6 tagged MBP_PrA/G in Escherichia coli

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   MBP PrA/G-H6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_2FLAG-AKAP79 WT

    Plasmid

    #183450

    Purpose

    Expression of human wild-type AKAP79

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLifeAct-HyPer7

    Plasmid

    #136464

    Purpose

    Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

    Plasmid

    #85434

    Purpose

    Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Pembrolizumab
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LAMP1-Venus

    Plasmid

    #234750

    Purpose

    Mammalian expression of lysosome marker LAMP1-Venus

    Depositor

    Nevin Lambert

    Article

    Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

    Insert

    LAMP1-Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. HC-M

    Plasmid

    #173481

    Purpose

    An rrnB P1-based GFP ATP reporter in E. coli

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

    Insert

    GFP-mut2
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    rrnB P1

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIS0

    Plasmid

    #12178

    Purpose

    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

    Depositor

    David Bartel

    Article

    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-ACVR2B

    Plasmid

    #23401

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ACVR2B (ACVR2B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    A333G, 1MM

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. 6xHis-SNAP-PI(3,4)P2 Probe

    Plasmid

    #211511

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    TAPP1 cPHx3
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-ALK-V5/HIS

    Plasmid

    #200115

    Purpose

    Expression of human ALK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ALK receptor tyrosine kinase (ALK Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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