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pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pKTrfp

Plasmid

#59772

Purpose

pKT230 derivative; PBAD_rfp; cmR

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Pa14Csy4

Plasmid

#41091

Depositor

Jennifer Doudna

Article

Haurwitz et al Science. 2010 Sep 10;329(5997):1355-8.

Insert

Csy4

Tags

His6-MBP

Expression

Bacterial

Mutation

Wild type

Promoter

T7

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pIRE4-Bpa

Plasmid

#155342

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

humanized EBpaRS/BstYam

Expression

Mammalian

Promoter

PGK/U6

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAT201

Plasmid

#134878

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPD0039_CROP-seq-CAR-Puro

Plasmid

#242532

Purpose

CAR T screening

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-BB CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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HO-hisG-URA3-hisG-poly-HO

Plasmid

#51663

Purpose

yeast plasmid for integration of target sequence at HO locus, URA3 selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pHCMV_MERS-CoV_S

Plasmid

#207293

Purpose

Mammalian expression of MERS coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MERS-CoV_S (S MERS coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP

Plasmid

#160697

Purpose

Expresses GFP in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-EBFP2

Plasmid

#14891

Purpose

Bacterial expression of EBFP2 blue fluorescent protein

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Has Service

Cloning Grade DNA

Insert

EBFP2

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1

Plasmid

#34707

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pDESTmycDDX17

Plasmid

#19876

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Neo

Plasmid

#99378

Purpose

Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

hNGN2-P2A-Neo (NEUROG2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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