164,247 results

1. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ac5-STABLE2-neo

   Plasmid

   #32426

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   Flag-mCherry-T2A-GFP-T2A-neo
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRUPATH Triple GaZ

   Plasmid

   #196053

   Purpose

   Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaz-RLuc8 (GNAZ Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_478

   Plasmid

   #179096

   Purpose

   BE Cas expression

   Depositor

   David Root, John Doench

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (SpG)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225241

   Purpose

   Mammalian expression of iPASnFR-H in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-STAT3-Y705F

   Plasmid

   #74434

   Purpose

   Expression of STAT3-Y705F

   Depositor

   Jie Chen

   Article

   Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

   Insert

   Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Mutations E16K, Y705F

   Promoter

   CMV

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

   Plasmid

   #85583

   Purpose

   cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

   Depositor

   Paul Sternberg

   Article

   Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   Prab-3-GAL4-SK(DBD)-VP64
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Prab-3-GAL4-SK(DBD)-VP64

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

   Plasmid

   #21845

   Depositor

   David Ron

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORI28

   Plasmid

   #71595

   Purpose

   EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

   Depositor

   Jan Kok, Todd Klaenhammer

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Type

   Empty backbone

   Use

   Integration vector for bacteria

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-SYN-mBaoJin-oPRE

    Plasmid

    #218683

    Purpose

    AAV vector plasmid expressing mBaoJin under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. plRL118

    Plasmid

    #163632

    Purpose

    Renilla luciferase reporter plasmid; Giles integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mycobacterial Optimized Promoter (MOP)

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEFIRES-P-EGFP-ADRP

    Plasmid

    #87157

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    mCherry-R TTGGTCACCTTCAGCTTGG

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag Rab12 wild type

    Plasmid

    #86787

    Purpose

    mammalian expression of Rab12

    Depositor

    Peter McPherson

    Article

    Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

    Insert

    Rab12 (RAB12 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5 FRT TO FLAG LRRK1

    Plasmid

    #232062

    Purpose

    Expresses FLAG-LRRK1 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. APEX2-OMM

    Plasmid

    #79056

    Purpose

    Soybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling protein

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Soybean APEX2
    

    Use

    Lentiviral

    Tags

    flag and last 31 amino acids of human MAVS

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

    Plasmid

    #179002

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-enCas12a-ABE8e-V106W

    Plasmid

    #193653

    Purpose

    Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-TadA*8e-V106W, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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