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pCold TDP43 WT

Plasmid

#27451

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa, GST, and His6

Expression

Bacterial

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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CNR1-DuET

Plasmid

#213213

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CNR1 (CNR1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDsRed1-CMV-Rat Arc-DsRed1-pA

Plasmid

#233049

Purpose

To Express a Rat Arc-DsRed fusion protein from a CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

Insert

rat Arc-DsRed fusion (Arc Rat)

Tags

DsRed1

Expression

Mammalian

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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TurboID-MCS MSCV-IRES-GFP

Plasmid

#207958

Purpose

Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

Depositor

Timothy Ley

Article

Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

Insert

TurboID

Use

Retroviral; Turboid biotin proximity ligase

Tags

TurboID

Expression

Mammalian

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-BICD2

Plasmid

#164626

Purpose

Expresses BICD2 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

BICD2 (BICD2 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_AAV

Plasmid

#119924

Purpose

All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

AAV

Tags

NLS-3xHA-NLS and NLS-NLS

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBetaActin-Halo-CAMSAP3

Plasmid

#191329

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

Halo-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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miniCMV-tdTomato-tDeg

Plasmid

#217521

Purpose

Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

tdTomato-tDeg fluorogenic protein

Expression

Mammalian

Promoter

miniCMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11-scarREF

Plasmid

#221184

Purpose

cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

None (same as cdGreen2) and PQ5 (cumate-inducible)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 4-1BB]

Plasmid

#218336

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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nuclear cpVenus control

Plasmid

#186790

Purpose

Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

nuclear cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Myc-Caspase-5

Plasmid

#214305

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe

Plasmid

#13787

Purpose

Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pSMP-YY1_2

Plasmid

#36358

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

YY1 (YY1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pCAGImC_Empty

Plasmid

#92015

Purpose

Empty backbone, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSEM319 - array co-injection fragment mosTI unc-119

Plasmid

#159823

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI unc-119

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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[#GS24 -LV] Syn1-GFP-OMP25

Plasmid

#233566

Purpose

Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

eGFP (eGFP Synthetic, Aequorea victoria)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB2

Plasmid

#23888

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pUB-sSypHer (RbcS-SypHer)

Plasmid

#84732

Purpose

Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

sSypHer

Tags

RbcS targeting sequence

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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