164,243 results

1. pX330-PITCh-MAPRE1

   Plasmid

   #207793

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-CEP135

   Plasmid

   #227286

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-cT3G-cERP2-PAX6a

   Plasmid

   #222576

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of PAX6a

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   PAX6a (PAX6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. cyto-iATPSnFR1.0

   Plasmid

   #102550

   Purpose

   cytoplasmic ATP sensor for mammalian cells

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. kiCAP-AAV-MyoAAV3F

   Plasmid

   #224447

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDHASW insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-N-RAM-d2tTA-TRE-mKate2

   Plasmid

   #140275

   Purpose

   N-RAM, the Npas4-dependent reporter

   Depositor

   Yingxi Lin

   Article

   Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

   Insert

   mKate2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pChREBP

   Plasmid

   #39235

   Depositor

   Isabelle Leclerc

   Article

   da Silva Xavier et al J Lipid Res. 2006 Nov;47(11):2482-91. Ep

   Insert

   ChREBP (Mlxipl Mouse)
   

   Use

   Tags

   cMyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pT2-shP53

   Plasmid

   #124261

   Purpose

   Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   shP53
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pMSCV-hygro-STING

    Plasmid

    #102598

    Purpose

    Express STING for IFN-beta luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. gTBEv3

    Plasmid

    #220619

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti EF1a-FLAG-dCas9-VPR

    Plasmid

    #114195

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-GFP-Sox11

    Plasmid

    #120387

    Purpose

    over expression of sox11 using infection

    Depositor

    Igor Ulitsky

    Article

    Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

    Insert

    sox11 (Sox11 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Lifeact-tdTomato

    Plasmid

    #64048

    Purpose

    Fluorescent reporter for F-actin labeling in living cells

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRF-SrpR

    Plasmid

    #49372

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    SrpR repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. c-myc promoter (Del 4)

    Plasmid

    #16604

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 4) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-4 (PvuII->PvuII). The first PvuII site is …

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205550

    Purpose

    Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Expression

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. phND2-N174

    Plasmid

    #31822

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    NeuroD2 (NEUROD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. LeGO-Cer2

    Plasmid

    #27338

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, Cerulean
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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